Manimaran Asokan, Manoharan Shanmugam, Neelakandan Mani
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar - 608002, Tamilnadu, India.
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):186-193. doi: 10.21010/ajtcam.v13i6.27. eCollection 2016.
The present study has evaluated the Emodin efficacy on the Akt, MAPK, ERK and DNMT expression pattern during 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinoma in golden Syrian hamsters, in order to explore its antitumor potential.
Oral tumors were developed in the buccal pouches of golden Syrian hamsters using the carcinogen, DMBA.
While the incidence of tumor formation was 100% in hamsters treated with DMBA alone, the tumor formation was not noticed in DMBA+ Emodin treated hamsters. Also, Emodin reduced the severity of precancerous pathological lesions such as dysplasia, in the hamsters treated with DMBA. Emodin administration corrected the abnormalities in the expression pattern of Akt, MAPK, ERK and DNMT in the buccal mucosa of hamsters treated with DMBA.
The present study thus suggests that the tumor preventive potential of Emodin is partly related to its modulating effect on the Akt, MAPK, ERK and DNMT expression pattern, as these molecular markers have a pivotal role in the process of cell proliferation, inflammation, invasion, and apoptosis.
本研究评估了大黄素对金黄叙利亚仓鼠在7,12-二甲基苯并[a]蒽（DMBA）诱导的口腔癌过程中Akt、丝裂原活化蛋白激酶（MAPK）、细胞外调节蛋白激酶（ERK）和DNA甲基转移酶（DNMT）表达模式的影响，以探索其抗肿瘤潜力。
使用致癌物DMBA在金黄叙利亚仓鼠的颊囊中诱发口腔肿瘤。
单独用DMBA处理的仓鼠肿瘤形成发生率为100%，而在DMBA+大黄素处理的仓鼠中未观察到肿瘤形成。此外，大黄素降低了DMBA处理的仓鼠癌前病理病变如发育异常的严重程度。大黄素给药纠正了DMBA处理的仓鼠颊黏膜中Akt、MAPK、ERK和DNMT表达模式的异常。
本研究因此表明，大黄素的肿瘤预防潜力部分与其对Akt、MAPK、ERK和DNMT表达模式的调节作用有关，因为这些分子标志物在细胞增殖、炎症、侵袭和凋亡过程中起关键作用。
