Manimaran Asokan, Manoharan Shanmugam
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, 608002, India.
Pathol Oncol Res. 2018 Jan;24(1):19-29. doi: 10.1007/s12253-017-0205-7. Epub 2017 Jan 31.
The aim of the present study is to focus the chemopreventive potential of Emodin during 7,12-dimethylbenz[a]anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Tumors were developed in the buccal pouches of golden Syrian hamsters by painting with 0.5% DMBA thrice a week for 14 weeks. The status of lipid peroxidation, antioxidants and detoxification agents were utilized as biochemical endpoints and the expression pattern of apoptotic proteins was employed as molecular endpoints in addition to the histopathological studies, to substantiate the anticancer potential of Emodin. Hamsters treated with DMBA + Emodin revealed mild to moderate precancerous lesions such as hyperplasia and dysplasia whereas 100% tumor formation was noticed in hamsters treated with DMBA alone. Also, Emodin treatment modulated the status of lipid peroxidation, antioxidants, phase I and II detoxification agents and apoptotic proteins in favor of the inhibition/reversal/suppression of the oral tumorigenesis in DMBA treated hamsters. The present study thus concludes that the chemopreventive potential of Emodin relies on its pro-apoptotic and antioxidant efficacy during DMBA induced hamster buccal pouch carcinogenesis.
本研究的目的是聚焦大黄素在7,12-二甲基苯并[a]蒽（DMBA）诱导的仓鼠颊囊癌变过程中的化学预防潜力。通过每周三次用0.5% DMBA涂抹金黄叙利亚仓鼠的颊囊，持续14周，诱导颊囊肿瘤发生。除了组织病理学研究外，脂质过氧化、抗氧化剂和解毒剂的状态被用作生化终点，凋亡蛋白的表达模式被用作分子终点，以证实大黄素的抗癌潜力。用DMBA + 大黄素处理的仓鼠显示出轻度至中度的癌前病变，如增生和发育异常，而仅用DMBA处理的仓鼠中观察到100%的肿瘤形成。此外，大黄素处理调节了脂质过氧化、抗氧化剂、I相和II相解毒剂以及凋亡蛋白的状态，有利于抑制/逆转/抑制DMBA处理的仓鼠中的口腔肿瘤发生。本研究因此得出结论，大黄素的化学预防潜力依赖于其在DMBA诱导的仓鼠颊囊癌变过程中的促凋亡和抗氧化功效。
