Zhou Liyi, Wang Qianqian, Tan Yi, Lang Matthew J, Sun Hongyan, Liu Xiaogang
College of Food Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan, 410004, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan, 410082, P. R. China.
Chemistry. 2017 Jun 27;23(36):8736-8740. doi: 10.1002/chem.201701365. Epub 2017 Jun 7.
Fluorophores with near-infrared emissions play a crucial role in numerous bioimaging and biosensing applications, owing to their deep penetration depths, low auto-fluorescence, and minimal tissue damages. Herein, the rational development of a new class of near-infrared fluorophores with bright one-photon and two-photon emissions at ≈740 nm, large Stokes shifts (≈80 nm), significant two-photon action absorption cross-section (≈185 GM at 820 nm), excellent water solubility, outstanding photostability, and low toxicity is reported. Their biological applications in mitochondrial labelling, deep tissue imaging, and H S detection in live cells and mice are also demonstrated. In addition, a rational design strategy for enlarging the Stokes shifts and enhancing two-photon emissions of these fluorophores is presented. These fluorophores will serve as a useful platform for developing novel imaging and sensing agents, and the design methodologies will inspire the molecular engineering of abundant high-performance near-infrared fluorophores.
具有近红外发射的荧光团在众多生物成像和生物传感应用中发挥着关键作用，这得益于它们的深层穿透深度、低自发荧光以及对组织的最小损伤。在此，报道了一类新型近红外荧光团的合理开发，其在≈740 nm处具有明亮的单光子和双光子发射、大斯托克斯位移（≈80 nm）、显著的双光子作用吸收截面（在820 nm处≈185 GM）、优异的水溶性、出色的光稳定性以及低毒性。还展示了它们在线粒体标记、深层组织成像以及活细胞和小鼠中H₂S检测方面的生物学应用。此外，提出了一种扩大这些荧光团斯托克斯位移并增强其双光子发射的合理设计策略。这些荧光团将成为开发新型成像和传感剂的有用平台，并且该设计方法将激发大量高性能近红外荧光团的分子工程。
