Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College , Tianjin 300192, P. R. China.
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, P. R. China.
ACS Appl Mater Interfaces. 2017 May 24;9(20):16880-16889. doi: 10.1021/acsami.7b03347. Epub 2017 May 11.
Because of the mounting prevalence of complicated infections induced by multidrug-resistant bacteria, it is imperative to develop innovative and efficient antibacterial agents. In this work, we design a novel polymeric micelle for simultaneous decorating of silver nanoparticles and encapsulating of curcumin as a combination strategy to improve the antibacterial efficiency. In the constructed combination system, silver nanoparticles were decorated in the micellar shell because of the in situ reduction of silver ions, which were absorbed by the poly(aspartic acid) (PAsp) chains in the shell. Meanwhile, natural curcumin was encapsulated into the poly(ε-caprolactone) (PCL) core of the micelle through hydrophobic interaction. This strategy could prevent aggregation of silver nanoparticles and improve the water solubility of curcumin at the same time, which showed enhanced antibacterial activity toward Gram-negative P.aeruginosa and Gram-positive S.aureus compared with sliver-decorated micelle and curcumin-loaded micelle alone, due to the cooperative antibacterial effects of the silver nanoparticles and curcumin. Furthermore, the achieved combinational micelles had good biocompatibility and low hemolytic activity. Thus, our study provides a new pathway in the rational design of combination strategy for efficiently preventing the ubiquitous bacterial infections.
由于多药耐药菌引起的复杂感染日益流行，因此必须开发创新和有效的抗菌剂。在这项工作中，我们设计了一种新型聚合物胶束，用于同时修饰银纳米粒子和封装姜黄素，以提高抗菌效率。在构建的组合系统中，由于银离子被壳中的聚天冬氨酸（PAsp）链原位还原，银纳米粒子被修饰在胶束壳中。同时，天然姜黄素通过疏水相互作用被包封到胶束的聚（ε-己内酯）（PCL）核中。这种策略可以防止银纳米粒子的聚集，同时提高姜黄素的水溶性，与单独的银修饰胶束和姜黄素负载胶束相比，对革兰氏阴性铜绿假单胞菌和革兰氏阳性金黄色葡萄球菌表现出更强的抗菌活性，这是由于银纳米粒子和姜黄素的协同抗菌作用。此外，所得到的组合胶束具有良好的生物相容性和低溶血活性。因此，我们的研究为合理设计联合策略提供了一条新途径，以有效预防普遍存在的细菌感染。
