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通过饱和转座对酵母基因组进行功能作图。

Functional mapping of yeast genomes by saturated transposition.

机构信息

Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.

Department of Biology, University of Fribourg, Fribourg, Switzerland.

出版信息

Elife. 2017 May 8;6:e23570. doi: 10.7554/eLife.23570.

DOI:10.7554/eLife.23570
PMID:28481201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5466422/
Abstract

Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput.

摘要

酵母是系统遗传学的强大模型。我们提出了一种通用、省时省力的方法，通过饱和转座子诱变结合高通量测序来对基因组进行功能探索。Yeast 中的饱和转座子分析（SATAY）允许在不破坏必需功能的情况下，一步定位所有转座子可以插入的遗传基因座。SATAY 特别适合发现各种条件下生长所必需的基因座。SATAY（1）揭示了单突变株和多突变株中的正、负遗传相互作用，（2）可以鉴定药物靶点，（3）不仅能检测必需基因，还能检测必需的蛋白质结构域，（4）既能产生无效等位基因，又能产生其他信息丰富的等位基因。在 SATAY 筛选雷帕霉素抗性突变体的过程中，我们鉴定出 Pib2（PhosphoInositide-Binding 2）是 TORC1 的主要调节因子。我们描述了位于 Pib2 两端的两种拮抗的 TORC1 激活和抑制活性。因此，SATAY 允许以空前的分辨率和通量轻松探索酵母基因组。

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2
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3
Conserved regulators of Rag GTPases orchestrate amino acid-dependent TORC1 signaling.Rag GTP酶的保守调节因子协调氨基酸依赖性的TORC1信号传导。
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4
Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice.可诱导转座子诱变技术可鉴定小鼠感染过程中的细菌适应性决定因素。
Nat Microbiol. 2025 May;10(5):1171-1183. doi: 10.1038/s41564-025-01975-z. Epub 2025 Mar 27.
5
Advances in CRISPR-enabled genome-wide screens in yeast.酵母中基于CRISPR的全基因组筛选技术进展
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