氨基糖苷-2'-O-甲基寡核糖核苷酸融合物的合成

Synthesis of Aminoglycoside-2'-O-Methyl Oligoribonucleotide Fusions.

作者信息

Granqvist Lotta, Kraszewski Andrzej, Tähtinen Ville, Virta Pasi

机构信息

Department of Chemistry, University of Turku, Vatselankatu 2, 20014 Turku, Finland.

Centre of New Technologies, University of Warsaw, Banacha 2c, 02 097 Warsaw, Poland.

出版信息

Molecules. 2017 May 8;22(5):760. doi: 10.3390/molecules22050760.

DOI:10.3390/molecules22050760

PMID:28481305

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6154110/

Abstract

Phosphoramidite building blocks of ribostamycin ( and ), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2'--methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2'--methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis.

摘要

合成了核糖霉素（和）的亚磷酰胺砌块，其可掺入寡核苷酸序列的任何位置。这些砌块与先前描述的新霉素修饰的固体支持物一起用于制备氨基糖苷-2'-O-甲基寡核糖核苷酸融合物。这些融合物用于夹住单链DNA序列（c-Myc启动子1的富含嘌呤的链）以形成三螺旋2'-O-甲基RNA/DNA杂交构建体。通过紫外熔解曲线分析研究了氨基糖苷部分稳定三螺旋构建体的潜力。