• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics.

作者信息

Karlovský P

机构信息

Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.

出版信息

J Theor Biol. 1988 May 7;132(1):7-14. doi: 10.1016/s0022-5193(88)80186-3.

DOI:10.1016/s0022-5193(88)80186-3
PMID:2848160
Abstract

A correction for results obtained by an analysis of DNA molecules partially cleaved with restriction endonuclease was suggested. The correction was proved on model data. Applications to (i) electron-microscopic analysis of singly cleaved molecules, (ii) partial digestion of a circular molecule followed by complete digestion with a second enzyme, (iii) systems with great cleavage differences, and (iv) partial cleavage of end-labelled molecules were discussed.

摘要

相似文献

1
Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics.
J Theor Biol. 1988 May 7;132(1):7-14. doi: 10.1016/s0022-5193(88)80186-3.
2
The EcoRI restriction endonuclease with bacteriophage lambda DNA. Kinetic studies.用噬菌体λDNA对EcoRI限制性内切核酸酶进行的动力学研究。
Biochem J. 1980 Nov 1;191(2):581-92. doi: 10.1042/bj1910581.
3
An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: its use to study the cleavage of phage lambda DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII.
Anal Biochem. 1983 Mar;129(2):446-56. doi: 10.1016/0003-2697(83)90575-4.
4
The EcoRI restriction endonuclease with bacteriophage lambda DNA. Equilibrium binding studies.
Biochem J. 1980 Nov 1;191(2):593-604. doi: 10.1042/bj1910593.
5
Kinetics of circular DNA molecule digestion by restriction endonuclease. Computation of kinetic constants from time dependence of fragment concentrations.限制性内切酶对环状DNA分子的消化动力学。根据片段浓度随时间的变化计算动力学常数。
Acta Biotheor. 1986;35(4):279-92. doi: 10.1007/BF00047098.
6
Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysis.大肠杆菌 EcoRI 核酸内切酶催化过程中的促进扩散。EcoRI 催化中的非特异性相互作用。
J Biol Chem. 1985 Oct 25;260(24):13130-7.
7
Stretching of single DNA molecules complexed with restriction endonuclease by Langmuir-Blodgett method.
Colloids Surf B Biointerfaces. 2005 Feb 25;40(3-4):123-6. doi: 10.1016/j.colsurfb.2004.10.025.
8
Ultraviolet irradiation of DNA: a way of generating partial digests for rapid restriction site mapping.DNA的紫外线照射：一种用于快速限制性酶切位点图谱分析的产生部分消化产物的方法。
Gene. 1986;41(1):129-34. doi: 10.1016/0378-1119(86)90276-3.
9
Asn141 is essential for DNA recognition by EcoRI restriction endonuclease.天冬酰胺141对于EcoRI限制性内切核酸酶识别DNA至关重要。
FEBS Lett. 1998 Oct 30;438(1-2):66-70. doi: 10.1016/s0014-5793(98)01274-5.
10
[Reaction of endonuclease EcoRI with DNA of the plasmid ColEI].
Mol Biol (Mosk). 1982 Jul-Aug;16(4):871-8.