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限制性内切酶对环状DNA分子的消化动力学。根据片段浓度随时间的变化计算动力学常数。

Kinetics of circular DNA molecule digestion by restriction endonuclease. Computation of kinetic constants from time dependence of fragment concentrations.

作者信息

Karlovský P

出版信息

Acta Biotheor. 1986;35(4):279-92. doi: 10.1007/BF00047098.

DOI:10.1007/BF00047098
PMID:3028020
Abstract

A model for kinetics of circular substrate cleavage by restriction endonuclease was formulated. The aim of the analysis of the model was to extract kinetic constants for all target sites from time-dependence of fragment concentration in reaction products. That was proved to be possible for molecules with an odd number of fragments only. A symmetry of the molecules with an even number of fragment is the cause. A solution for molecules with an odd number of fragments was found and methods for dealing with the other molecules were suggested.

摘要

构建了一个限制性内切酶切割环状底物动力学模型。该模型分析的目的是根据反应产物中片段浓度随时间的变化来提取所有靶位点的动力学常数。事实证明，这仅对具有奇数个片段的分子可行。具有偶数个片段的分子的对称性是原因所在。找到了具有奇数个片段的分子的解决方案，并提出了处理其他分子的方法。

相似文献

1
Kinetics of circular DNA molecule digestion by restriction endonuclease. Computation of kinetic constants from time dependence of fragment concentrations.限制性内切酶对环状DNA分子的消化动力学。根据片段浓度随时间的变化计算动力学常数。
Acta Biotheor. 1986;35(4):279-92. doi: 10.1007/BF00047098.
2
Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics.
J Theor Biol. 1988 May 7;132(1):7-14. doi: 10.1016/s0022-5193(88)80186-3.
3
The SalGI restriction endonuclease. Mechanism of DNA cleavage.SalGI限制性内切核酸酶。DNA切割机制。
Biochem J. 1982 Apr 1;203(1):85-92. doi: 10.1042/bj2030085.
4
Catalytic properties of the HhaII restriction endonuclease.HhaII限制性内切核酸酶的催化特性
J Biol Chem. 1985 Dec 5;260(28):15345-51.
5
Preferential site-dependent cleavage by restriction endonuclease PstI.限制性内切酶PstI的位点依赖性优先切割
Nucleic Acids Res. 1982 Feb 11;10(3):993-1007. doi: 10.1093/nar/10.3.993.
6
Optimization of the EcoRI-activity of EcoRI endonuclease.
FEBS Lett. 1978 Jun 15;90(2):341-4. doi: 10.1016/0014-5793(78)80400-1.
7
Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.用限制性内切酶Eco RI切割腺病毒DNA的动力学研究。
Nucleic Acids Res. 1976 Dec;3(12):3255-69. doi: 10.1093/nar/3.12.3255.
8
Transient cleavage kinetics of the Eco RI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change.在脉冲淬灭流动装置中测量的Eco RI限制性内切酶的瞬时切割动力学：酶浓度依赖性活性变化。
Nucleic Acids Res. 1981 Jul 24;9(14):3483-90. doi: 10.1093/nar/9.14.3483.
9
Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.通过限制性内切核酸酶切割对闭环DNA进行图谱分析以及通过琼脂糖凝胶电泳进行校准。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):851-5. doi: 10.1073/pnas.74.3.851.
10
[Reaction of endonuclease EcoRI with DNA of the plasmid ColEI].
Mol Biol (Mosk). 1982 Jul-Aug;16(4):871-8.

本文引用的文献

1
A Note on the Kinetics of Enzyme Action.关于酶作用动力学的注释
Biochem J. 1925;19(2):338-9. doi: 10.1042/bj0190338.
2
The influence of sequences adjacent to the recognition site on the cleavage of oligodeoxynucleotides by the EcoRI endonuclease.识别位点附近序列对EcoRI核酸内切酶切割寡脱氧核苷酸的影响。
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3
Supercoiling and the mechanism of restriction endonucleases.超螺旋与限制性核酸内切酶的作用机制
Eur J Biochem. 1984 Jan 16;138(2):275-80. doi: 10.1111/j.1432-1033.1984.tb07912.x.
4
Site-dependent cleavage of pBR322 DNA by restriction endonuclease HinfI.限制性内切酶HinfI对pBR322 DNA的位点依赖性切割。
Nucleic Acids Res. 1983 Jun 25;11(12):4109-26. doi: 10.1093/nar/11.12.4109.
5
An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: its use to study the cleavage of phage lambda DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII.
Anal Biochem. 1983 Mar;129(2):446-56. doi: 10.1016/0003-2697(83)90575-4.
6
Preferential site-dependent cleavage by restriction endonuclease PstI.限制性内切酶PstI的位点依赖性优先切割
Nucleic Acids Res. 1982 Feb 11;10(3):993-1007. doi: 10.1093/nar/10.3.993.
7
Structural junctions in DNA: the influence of flanking sequence on nuclease digestion specificities.DNA中的结构连接：侧翼序列对核酸酶消化特异性的影响。
Nucleic Acids Res. 1985 Jun 25;13(12):4445-67. doi: 10.1093/nar/13.12.4445.
8
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.用EcoRI限制性内切酶切割噬菌体λ DNA的研究。
J Mol Biol. 1975 Jan 25;91(3):315-28. doi: 10.1016/0022-2836(75)90383-6.
9
Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.用限制性内切酶Eco RI切割腺病毒DNA的动力学研究。
Nucleic Acids Res. 1976 Dec;3(12):3255-69. doi: 10.1093/nar/3.12.3255.
10
Structures and mechanisms of DNA restriction and modification enzymes.
Q Rev Biophys. 1979 Aug;12(3):315-69. doi: 10.1017/s0033583500005461.