Bodnar R J, Clark J A, Cooper M L, Pasternak G W
George C. Cotzias Laboratory of Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY.
Life Sci. 1988;43(21):1697-700. doi: 10.1016/0024-3205(88)90480-8.
Opiate receptors have been identified within the striatum and some have been localized presynaptically to nigrostriatal neurons. Using unilateral ablative lesions of the substantia nigra, we examined binding in the ipsilateral and contralateral striata. Lesions significantly lowered both 3H[D-Ala2,MePhe4,Gly(ol)5]enkephalin (DAGO) and 3H[D-Ala2,Leu5]enkephalin (DADL) binding. The inclusion of competitors in these assays revealed a decrease in both mu1 and mu2 receptors. Mu1 binding was slightly more sensitive to the lesioning than mu2 binding. Selective mu1 and mu2 binding assays supported these observations. No change in delta binding was observed in the lesioned striata. These studies raise the possibility that both mu1 and mu2, but not delta, receptors are localized presynaptically on nigrostriatal neurons.
在纹状体内已鉴定出阿片受体，其中一些已定位在黑质纹状体神经元的突触前。我们利用黑质的单侧毁损性损伤，检查了同侧和对侧纹状体中的结合情况。损伤显著降低了³H[D-丙氨酸²，甲硫氨酸苯丙氨酸⁴，甘氨酸（醇）⁵]脑啡肽（DAGO）和³H[D-丙氨酸²，亮氨酸⁵]脑啡肽（DADL）的结合。在这些测定中加入竞争剂后发现μ₁和μ₂受体均减少。μ₁结合比对损伤的敏感性略高于μ₂结合。选择性μ₁和μ₂结合测定支持了这些观察结果。在损伤的纹状体中未观察到δ结合的变化。这些研究提出了μ₁和μ₂受体而非δ受体可能定位在黑质纹状体神经元突触前的可能性。
