[致病性沙粒病毒的多聚腺苷酸阳性和多聚腺苷酸阴性信使核糖核酸的模板活性]

[Template activity of poly(A+) and poly(A-) messenger RNA of pathogenic arenaviruses].

作者信息

Scheslenok E P, Stel'makh T A, Shkolina T V, Lukashevich I S

出版信息

Mol Gen Mikrobiol Virusol. 1988 Jul(7):29-31.

Abstract

The total RNA from cells infected with Machupo and Lassa viruses as well as poly(A+) and poly(A-) fractions of the RNA were translated in the cell-free protein synthesizing system from rabbit reticulocytes. The translated products were treated with specific antibodies and analyzed in polyacrylamide gel electrophoresis. Only poly(A-) fraction of RNA coded for the synthesis of NP protein in vitro. The mRNAs for NP protein of Machupo and Lassa viruses are supposed to contain no poly(A) sequences at 3'end, or if they really do, the size of the sequences is not adequate for binding with oligo(dT)-cellulose.

摘要

用来自兔网织红细胞的无细胞蛋白质合成系统翻译感染马丘波病毒和拉沙病毒的细胞的总RNA以及RNA的聚腺苷酸（poly(A+)）和非聚腺苷酸（poly(A-)）组分。将翻译产物用特异性抗体处理，并在聚丙烯酰胺凝胶电泳中进行分析。仅RNA的poly(A-)组分在体外编码核蛋白（NP）的合成。推测马丘波病毒和拉沙病毒NP蛋白的信使核糖核酸（mRNA）在3'端不包含聚腺苷酸序列，或者即便它们确实含有，这些序列的长度也不足以与寡聚（dT）-纤维素结合。