Lukashevich I S, Stelmakh T A, Stchesljenok E P, Shkolina T V
Arch Virol. 1987;92(3-4):315-9. doi: 10.1007/BF01317488.
RNA from Machupo virus infected cells was centrifuged in a linear sucrose gradient and RNAs from gradient fractions were tested separately for template activity in a cell-free protein synthesizing system from rabbit reticulocytes. Fraction 15-16 S programmed the synthesis of protein that migrated in SDS-polyacrylamide gel like the nucleocapsid protein of a purified virus. The synthesis of virus glycoproteins was not detected in the system.
将来自感染马丘波病毒的细胞的RNA在线性蔗糖梯度中离心，然后分别检测梯度级分中的RNA在兔网织红细胞无细胞蛋白质合成系统中的模板活性。15 - 16S级分指导合成的蛋白质在SDS - 聚丙烯酰胺凝胶中的迁移情况与纯化病毒的核衣壳蛋白相似。在该系统中未检测到病毒糖蛋白的合成。
