Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Beijing Key Lab of Bioprocess, National Energy R&D Center for Biorefinery, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
Bioresour Technol. 2017 Aug;238:575-581. doi: 10.1016/j.biortech.2017.04.092. Epub 2017 Apr 27.
In this study, a new pretreatment strategy for lignocellulosic was developed using a fully recyclable solid acid, Toluenesulfonic acid (p-TsOH). A combined hydrolysis factor (CHF) as a pretreatment severity was used to balance enzymatic saccharification and the structural characteristics of lignin. The results from degradation of carbohydrates, enzymatic hydrolysis of cellulose and characterization of lignin by FT-IR, P NMR, GPC, 2D-HSQC NMR indicated that a CHF of approximately 3.90 was the optimal pretreatment severity to facilitate enzymatic saccharification and the potential serviceability of lignin. Then approximately 90% of the xylan was removed to result in a reasonable sugar yield of 76%. Residual lignin showed low molecular weight (Mw, 5783g/mol), narrow polydispersities (Mw/Mn, 1.10) and high content of phenolic hydroxyl groups (3.702mmol/g); it may be a potential feedstock for phenol monomer and polymeric materials production. In short, this process was regarded as a promising approach to achieve an efficient conversion of lignocellulosic biomass to sugar products and lignin-based materials.
在这项研究中，使用一种完全可回收的固体酸对木质纤维素进行了新的预处理策略，即对甲苯磺酸（p-TsOH）。采用综合水解因子（CHF）作为预处理强度来平衡酶解和木质素的结构特征。碳水化合物降解、纤维素的酶水解以及通过傅里叶变换红外光谱（FT-IR）、磷核磁共振（P NMR）、凝胶渗透色谱（GPC）、二维异核单量子相关核磁共振（2D-HSQC NMR）对木质素进行的表征结果表明，CHF 约为 3.90 是促进酶解和木质素潜在适用性的最佳预处理强度。然后，大约 90%的木聚糖被去除，从而产生了 76%的合理糖产量。残留木质素表现出低分子量（Mw，5783g/mol）、较窄的多分散性（Mw/Mn，1.10）和高含量的酚羟基（3.702mmol/g）；它可能是酚单体和聚合材料生产的潜在原料。简而言之，该工艺被认为是实现木质纤维素生物质向糖产品和木质素基材料高效转化的有前途的方法。
