Comparison of enzymatic saccharification and lignin structure of masson pine and poplar pretreated by p-Toluenesulfonic acid.

p-Toluenesulfonic acid (p-TsOH) with the hydrotropic and recyclable properties is widely used for rapid remove of lignin from lignocelluloses at low temperature (<100 °C). In this work, both softwood masson pine and hardwood poplar were pretreated with p-TsOH under different conditions and then subjected to enzymatic hydrolysis to compare the effect of p-TsOH pretreatment on their saccharification and lignin structure. Results showed p-TsOH has sensitive selectivity to lignin structure during pretreatment. Around 95% of lignin in poplar can be dissolved at 80 °C within 30 min, while for masson pine, the delignification is only 50%. Following enzymatic hydrolysis with cellulase loading of 20 FPU/g-cellulose for 72 h, the highest sugar yield of pretreated poplar and masson pine is 92.13% and 29.46%, respectively, which indicates that p-TsOH pretreatment alone works well with hardwoods (poplar). Structural analysis of removed lignin implies that p-TsOH mainly results in the cleavage of β-aryl ether bonds of lignin side chains, and the aromatic structure of lignin keeps intact. p-TsOH pretreatment shows the key advantages of low cost and rapid delignification for highly enzymatic saccharification, and provides a promising and green pathway for the development of low cost and sustainable bio-based products for developing a bio-based economy.