Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China.
Bioresour Technol. 2017 Aug;238:602-608. doi: 10.1016/j.biortech.2017.04.058. Epub 2017 Apr 19.
Biofilm based microalgal cultivation has recently received great attention because of its low water requirement and harvesting cost. However, the contradiction between microalgal attachment and harvesting still hinders the development of this technology. Therefore, in this study the most readily available and inexpensive shifting sand was used as attached substrate for microalgal (Microcoleus vaginatus) biofilm cultivation under different water conditions. After the inoculation, a stable and easily peeled microalgal biofilm formed through filamentous binding and exopolysaccharide cementing. In general, microalgal biomass, photosynthetic activity and exopolysaccharide accumulation were all significantly affected by the cultivation time, water content and their interaction (P<0.001). According to the maximal photosynthetic activity and microalgal productivity, cultivation time of microalgal biofilm on sand surface should be controlled around 15-25days, with water content at 10%. Based on the biofilm cultivation system, microalgal biomass yield reached up to 11gm eventually on the sand surface.
基于生物膜的微藻培养因其耗水和收获成本低而受到广泛关注。然而，微藻附着和收获之间的矛盾仍然阻碍了这项技术的发展。因此，本研究采用最易得且最便宜的移沙作为附着基质，在不同的水分条件下进行微藻（微鞘藻）生物膜的培养。接种后，通过丝状结合和胞外多糖胶结形成稳定且易于剥离的微藻生物膜。总的来说，微藻生物量、光合作用活性和胞外多糖积累都受到培养时间、含水量及其相互作用的显著影响（P<0.001）。根据最大光合作用活性和微藻生产力，微藻生物膜在沙面上的培养时间应控制在 15-25 天左右，含水量为 10%。基于生物膜培养系统，最终在沙面上获得了高达 11g/m2 的微藻生物量。
