Deng M H, Xu J, Cai H X, Fang W, Long X
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China.
2nd Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China [Present address: Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Chongqing Medical University, Chongqing 400016, China].
Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 May 9;52(5):274-277. doi: 10.3760/cma.j.issn.1002-0098.2017.05.003.
To investigate the effect of temporomandibular joint (TMJ) disc perforation on expression of type Ⅰ collagen in TMJ disc cells. The fibroblastic-like cells from the surgical removed TMJ disc tissue (disc perforation or TMJ condyle hyperplasia) were cultured. The cultured cells were identified as fibroblastic-like cells by toluidine blue and immunofluorescence staining. The expression of type Ⅰ collagen was detected with Western blotting and the content of type Ⅰ collagen was examined by enzyme linked immunosorbent assay (ELISA). Fibroblastic-like cells were cultured from TMJ disc cells and the controls. The collagen-Ⅰ and collagen-Ⅱ were positive in both toluidine blue and immunofluorescence staining. In Western blotting, the expression of typeⅠcollagen in cells from joints with disc perforation was lower than that from normal joints. The content of collagen-Ⅰ was (1.62±0.52) μg/L from controls, and (0.85±0.33) μg/L from disc perforation respectively (0.0134). The disc cells from TMJ with disc perforation expressed lower type Ⅰ collagen than that from controls, which may be related to the lower content of collagen-Ⅰ in TMJ disc and the formation of TMJ disc perforation.
探讨颞下颌关节（TMJ）盘穿孔对TMJ盘细胞中Ⅰ型胶原蛋白表达的影响。培养手术切除的TMJ盘组织（盘穿孔或TMJ髁突增生）中的成纤维样细胞。通过甲苯胺蓝和免疫荧光染色将培养的细胞鉴定为成纤维样细胞。采用蛋白质印迹法检测Ⅰ型胶原蛋白的表达，并用酶联免疫吸附测定（ELISA）检测Ⅰ型胶原蛋白的含量。从TMJ盘细胞和对照中培养成纤维样细胞。甲苯胺蓝和免疫荧光染色中胶原蛋白-Ⅰ和胶原蛋白-Ⅱ均呈阳性。在蛋白质印迹法中，盘穿孔关节细胞中Ⅰ型胶原蛋白的表达低于正常关节细胞。对照组胶原蛋白-Ⅰ的含量分别为（1.62±0.52）μg/L，盘穿孔组为（0.85±0.33）μg/L（P=0.0134）。TMJ盘穿孔时的盘细胞表达的Ⅰ型胶原蛋白低于对照组，这可能与TMJ盘中胶原蛋白-Ⅰ含量降低及TMJ盘穿孔的形成有关。
