Landesberg R, Takeuchi E, Puzas J E
New York University, College of Dentistry, Department of Basic Sciences and Surgical Sciences, NY 10010, USA.
Arch Oral Biol. 1996 Aug-Sep;41(8-9):761-7. doi: 10.1016/s0003-9969(96)00068-4.
The cellular and collagenous components of the bovine temporomandibular joint (TMJ) disc have been isolated and analysed. In the central regions of the disc, significant amounts of type I, II, IX and XII collagen were found. The identity of these molecules was verified with collagenase digestions, Western blot analysis and Northern blot analysis (for type II collagen). Cells isolated from the TMJ disc synthesized alkaline phosphatase, proteoglycans and collagen in culture; however, the basal rate of synthesis for these molecules was lower than that for isolated osteoblasts, articular and growthplate chondrocytes. The TMJ disc cells proliferated more rapidly in culture than osteoblasts or chondrocytes. Transforming growth factor-beta stimulated proliferation by 250%, whereas prostaglandin E2 had no effect.
牛颞下颌关节（TMJ）盘的细胞和胶原成分已被分离和分析。在关节盘的中央区域，发现了大量的I型、II型、IX型和XII型胶原。通过胶原酶消化、蛋白质印迹分析和Northern印迹分析（针对II型胶原）验证了这些分子的特性。从TMJ盘分离出的细胞在培养中合成碱性磷酸酶、蛋白聚糖和胶原；然而，这些分子的基础合成率低于分离出的成骨细胞、关节软骨细胞和生长板软骨细胞。TMJ盘细胞在培养中的增殖速度比成骨细胞或软骨细胞更快。转化生长因子-β使增殖增加250%，而前列腺素E2则没有作用。
