Perumal Govindaraj, Pappuru Sreenath, Chakraborty Debashis, Maya Nandkumar A, Chand Dillip Kumar, Doble Mukesh
Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600 036, India.
Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:1196-1204. doi: 10.1016/j.msec.2017.03.200. Epub 2017 Mar 23.
This study is aimed to develop curcumin (Cur) incorporated electrospun nanofibers of a blend of poly (lactic acid) (PLA) and hyperbranched polyglycerol (HPG) for wound healing applications. Both the polymers are synthesized and fabricated by electrospinning technique. The produced nanofibers were characterized by Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric Analysis (TGA). Electrospun scaffolds (PLA/HPG/Cur) exhibits very high hydrophilicity, high swelling and drug uptake and promotes better cell viability, adhesion and proliferation when compared to PLA/Cur electrospun nanofibers. Biodegradation study revealed that the morphology of the nanofibers were unaffected even after 14days immersion in Phosphate Buffered Saline. In vitro scratch assay indicates that migration of the cells in the scratch treated with PLA/HPG/Cur is complete within 36h. These results suggest that PLA/HPG/Cur nanofibers can be a potential wound patch dressing for acute and chronic wound applications.
本研究旨在开发一种用于伤口愈合的、含有姜黄素（Cur）的聚乳酸（PLA）与超支化聚甘油（HPG）共混物的电纺纳米纤维。两种聚合物均通过静电纺丝技术合成与制备。所制备的纳米纤维通过扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、差示扫描量热法（DSC）和热重分析（TGA）进行表征。与PLA/Cur电纺纳米纤维相比，电纺支架（PLA/HPG/Cur）表现出非常高的亲水性、高溶胀性和药物吸收性，并能促进更好的细胞活力、黏附及增殖。生物降解研究表明，即使在磷酸盐缓冲盐水中浸泡14天后，纳米纤维的形态也未受影响。体外划痕试验表明，用PLA/HPG/Cur处理的划痕中的细胞在36小时内迁移完成。这些结果表明，PLA/HPG/Cur纳米纤维可成为用于急性和慢性伤口应用的潜在伤口贴片敷料。
