Gong Cheng-Bin, Wei Yu-Bo, Liu Lan-Tao, Zheng An-Xun, Yang Yue-Hong, Chow Cheuk-Fai, Tang Qian
The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Department of Science and Environmental Studies, The Education University of Hong Kong, Hong Kong.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:568-578. doi: 10.1016/j.msec.2017.03.135. Epub 2017 Mar 18.
This paper reports a photoresponsive hollow molecularly imprinted polymer for the determination of trace triamterene in biological sample. The photoresponsive hollow molecularly imprinted polymer was prepared on sacrificial silica microspheres via surface imprinting technique through atom transfer radical polymerization using a novel water-soluble azobenzene derivative, 4-[(4-methacryloyloxy)phenylazo]-3,5-dimethyl benzenesulfonic acid, as the functional monomer, and the sacrificial silica core was subsequently removed using HF etching method with 1.25vol.% HF ethanolic solution. The morphologies and properties of the photoresponsive hollow molecularly imprinted polymer were further characterized and compared systematically with the corresponding photoresponsive surface molecularly imprinted polymer. Compared with surface imprinted polymer, the hollow material displayed higher binding capacity, better recognition ability, faster mass-transfer rate, and larger isomerization rate constants toward triamterene. The static binding properties of the imprinted materials were investigated under three irradiation conditions. The photoresponsive hollow molecularly imprinted polymer showed better specificity toward triamterene than its structural analogues (folic acid and caffeine) as examined by UV-vis and HPLC. The photoresponsive hollow molecularly imprinted polymer was utilized for the determination of trace triamterene in biological samples (human urine and serum) with advantages of simple sample pre-treatment, good recovery and good sensitivity.
本文报道了一种用于测定生物样品中痕量氨苯蝶啶的光响应性中空分子印迹聚合物。通过表面印迹技术，以新型水溶性偶氮苯衍生物4-[(4-甲基丙烯酰氧基)苯基偶氮]-3,5-二甲基苯磺酸为功能单体，在牺牲二氧化硅微球上通过原子转移自由基聚合制备了光响应性中空分子印迹聚合物，随后使用1.25体积%的氢氟酸乙醇溶液通过氢氟酸蚀刻法去除牺牲二氧化硅核。对光响应性中空分子印迹聚合物的形态和性能进行了进一步表征，并与相应的光响应性表面分子印迹聚合物进行了系统比较。与表面印迹聚合物相比，中空材料对氨苯蝶啶表现出更高的结合容量、更好的识别能力、更快的传质速率和更大的异构化速率常数。在三种光照条件下研究了印迹材料的静态结合性能。通过紫外可见光谱和高效液相色谱检测，光响应性中空分子印迹聚合物对氨苯蝶啶的特异性优于其结构类似物（叶酸和咖啡因）。光响应性中空分子印迹聚合物用于测定生物样品（人尿液和血清）中的痕量氨苯蝶啶，具有样品前处理简单、回收率高和灵敏度好的优点。
