Shoja Yalda, Rafati Amir Abbas, Ghodsi Javad
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O. Box 65174, Hamedan, Iran.
Department of Physical Chemistry, Faculty of Chemistry, Bu-Ali Sina University, P.O. Box 65174, Hamedan, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:637-645. doi: 10.1016/j.msec.2017.03.155. Epub 2017 Mar 20.
A novel, highly selective and sensitive voltammetric bi-enzyme biosensor for sensing dopamine (DA), in presence of HO which resulted as d-alanine enzymatic oxidation, was fabricated on the basis of simultaneously electrostatically immobilization of d-amino acid oxidase (DAAO) and hemoglobin (Hb) on MnO nanoparticles (MnO NPs) enriched poly thiophene (PTh). Cyclic voltammetry (CV), technique was applied for electropolymerization of thiophene on glassy carbon electrode (GCE) surface and MnO NPs dispersed on PTh network by soaking PTh/GCE in potassium permanganate (KMnO) solution. Excellent catalytic properties and large surface area of MnO NPs/PTh composite caused it was used as an enzymes immobilization host in this developed bi-enzyme biosensor. The developed biosensor was characterized by scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), and CV. The performance of the modified bi-enzyme biosensor was investigated in terms of its response time, detection limit, sensitivity, stability and selectivity in a lab environment. The composite of DAAO-Hb/MnO NPs/PTh to construct a bi-enzyme biosensor in this study showed a linear response with DA in the concentration range of 0.04-9.0μM with R-squared value of 0.994 (for S/N=3) and its sensitivity and detection limite were about 12.801μA/μM and 41nM respectively. Also this bi-enzyme biosensors exhibited high selectivity, rapid response (5s) and long-term stability (42days). At the end, the proposed biosensor was applied successfully in human serum as real sample.
基于将d - 氨基酸氧化酶（DAAO）和血红蛋白（Hb）同时静电固定在富含MnO纳米颗粒（MnO NPs）的聚噻吩（PTh）上，制备了一种新型、高选择性且灵敏的伏安双酶生物传感器，用于在由d - 丙氨酸酶促氧化产生的HO存在下传感多巴胺（DA）。采用循环伏安法（CV）技术在玻碳电极（GCE）表面电聚合噻吩，并通过将PTh / GCE浸泡在高锰酸钾（KMnO）溶液中使MnO NPs分散在PTh网络上。MnO NPs / PTh复合材料优异的催化性能和大表面积使其被用作这种新型双酶生物传感器中的酶固定载体。通过扫描电子显微镜（SEM）、能量色散X射线分析（EDX）和CV对所制备的生物传感器进行了表征。在实验室环境中，从响应时间、检测限、灵敏度、稳定性和选择性等方面对修饰后的双酶生物传感器的性能进行了研究。本研究中构建的用于双酶生物传感器的DAAO - Hb / MnO NPs / PTh复合材料对DA在0.04 - 9.0μM浓度范围内呈现线性响应，R平方值为0.994（S / N = 3），其灵敏度和检测限分别约为12.801μA / μM和41nM。此外，这种双酶生物传感器还表现出高选择性、快速响应（5s）和长期稳定性（42天）。最后，所提出的生物传感器成功应用于人体血清作为实际样品。
