Klinedinst A F, Baldwin M L, Ness P M
Johns Hopkins Hospital Blood Bank, Baltimore, Maryland.
Transfusion. 1988 Nov-Dec;28(6):563-5. doi: 10.1046/j.1537-2995.1988.28689059032.x.
The detection of antibody to cytomegalovirus (CMV) in donor sera is one effective method for the prevention of posttransfusion CMV infection in seronegative recipients. A passive latex agglutination test (CMV Scan, Hynson, Wescott & Dunning, Baltimore, MD) has been shown to be an acceptable method of screening sera from the blood donor population. The procedure for this test, however, does not permit the testing of stored donor blood products. To evaluate the feasibility of testing blood components at various times during storage, the authors examined 25 CMV-positive and 25-CMV negative samples of CPDA-1 plasma from 50 units each of platelets and red cells. Plasma samples from platelets stored at 22 degrees C were tested on each of the 5 days of storage. Samples from red cell units stored at 2 to 6 degrees C were tested on storage Days 1, 2, 4, 6, 8, 10, 12, and 14. Of 250 tests done on plasma from platelet units, there were 123 true-positive and 123 true-negative results (sensitivity and specificity, 98.4%). Of 400 tests on plasma from red cell units, there were 200 true-positive and 198 true-negative results (sensitivity, 100%; specificity, 99.5%). These data show that the CMV Scan test can be used reliably to test segments of CPDA-1 plasma from platelets stored for up to 5 days and from red cells stored for up to 14 days.
检测供体血清中的巨细胞病毒（CMV）抗体是预防血清反应阴性受血者输血后CMV感染的一种有效方法。被动乳胶凝集试验（CMV Scan，Hynson，Wescott & Dunning，巴尔的摩，马里兰州）已被证明是一种可接受的筛查献血人群血清的方法。然而，该试验的操作程序不允许对储存的供体血液制品进行检测。为了评估在储存期间不同时间检测血液成分的可行性，作者检测了来自50个单位血小板和红细胞的25份CMV阳性和25份CMV阴性的CPDA-1血浆样本。在储存的5天里，每天都对储存在22摄氏度的血小板的血浆样本进行检测。对储存在2至6摄氏度的红细胞单位的样本在储存第1、2、4、6、8、10、12和14天进行检测。在对血小板单位血浆进行的250次检测中，有123次真阳性和123次真阴性结果（敏感性和特异性为98.4%）。在对红细胞单位血浆进行的400次检测中，有200次真阳性和198次真阴性结果（敏感性为100%；特异性为99.5%）。这些数据表明，CMV Scan试验可可靠地用于检测储存长达5天的血小板和储存长达14天的红细胞的CPDA-1血浆片段。
