Wang Xinrong, Gong Ke, Li Haiyan, Wang Congyi, Qu Chaoyi, Li Hui
Department of Ophthalmology, The Fourth Hospital of Xi'an, Xi'an, Shaanxi 710004, China.
Department of Endocrinology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.
J Ophthalmol. 2017;2017:6896390. doi: 10.1155/2017/6896390. Epub 2017 Apr 5.
. The pressure-induced axonal injury of the vulnerable ONH has led many researchers to view glaucoma from the perspective of the genetic basis of the angle of the ONH. However, genetic studies on POAG from this perspective are limited. . Microarray dataset GSE45570 of the ONH of healthy individuals and POAG patients were downloaded from the Gene Expression Omnibus. After screening for the DEGs using the limma package, enrichment analysis was performed using DAVID. The DEG interaction network was constructed using cancer spider at BioProfiling.de. Thereafter, DEG-related TFs were predicted using TRANSFAC, and TF-DEG regulatory networks were visualized using Cytoscape. . Thirty-one DEGs were identified including 11 upregulated and 20 downregulated DEGs. Thereafter, gene ontology terms of nucleosome assembly, sensory perception and cognition, and pathway of signaling by GPCR were found to be enriched among the DEGs. Furthermore, DEG interaction and TF-DEG networks were constructed. NEUROD1 was present in both the DEG network and the TF-DEG network as the node with the highest degree and was predicted as a marker gene in the ONH of patients with POAG. . NEUROD1 may contribute greatly to the ONH of patients with POAG and was found to be involved in eye development and diseases.
视盘（ONH）易受压力诱导的轴突损伤，这使得许多研究人员从视盘角度的遗传基础来审视青光眼。然而，从这个角度对原发性开角型青光眼（POAG）进行的基因研究有限。从基因表达综合数据库下载了健康个体和POAG患者视盘的微阵列数据集GSE45570。使用limma软件包筛选差异表达基因（DEGs）后，利用DAVID进行富集分析。使用BioProfiling.de网站的cancer spider构建DEG相互作用网络。此后，使用TRANSFAC预测与DEG相关的转录因子（TFs），并使用Cytoscape可视化TF-DEG调控网络。共鉴定出31个DEGs，其中11个上调，20个下调。此后，发现核小体组装、感官知觉和认知的基因本体术语以及GPCR信号通路在DEGs中富集。此外，构建了DEG相互作用网络和TF-DEG网络。NEUROD1作为度数最高的节点同时存在于DEG网络和TF-DEG网络中，并被预测为POAG患者视盘中的一个标记基因。NEUROD1可能对POAG患者的视盘有很大影响，并且发现其参与眼睛发育和疾病。
