Li Sanpeng, Sun Zhihong, Deng Guanjun, Meng Xiaoqing, Li Wenjun, Ni Dapeng, Zhang Jiali, Gong Ping, Cai Lintao
Guangdong Key Laboratory of Nanomedicine, CAS Key Lab for Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China.
Biomater Sci. 2017 May 30;5(6):1122-1129. doi: 10.1039/c7bm00230k.
Targeted phototherapy and multi-modal imaging can effectively improve the therapeutic efficacy and reduce the side effects of theranostics. Herein, we constructed novel biocompatible cyanine dye IR808-conjugated hyaluronic acid nanoparticles (HAIR NPs) for photothermal therapy (PTT) with near-infrared fluorescence (FL) and photoacoustic (PA) dual-modal imaging. The nanoparticles formed stable nanostructures under aqueous conditions with uniform size distribution. The HAIR NPs were rapidly taken up by the human lung cancer cells A549 via CD44 (the hyaluronic acid receptor on the surface of tumor cells) receptor-mediated endocytosis. Upon laser irradiation, the HAIR NPs enabled good near-infrared fluorescence imaging and photoacoustic imaging in tumor-bearing mice. In addition, the tight nanostructure arising from the covalent link between HA and IR808 could significantly improve the light-thermal conversion efficiency of IR808. Under a low dose of laser power, the HAIR NPs presented more effective photothermal therapy for the suppression of tumor growth than free IR808 in vitro and in vivo. Overall, these results indicate that the HAIR NPs may be an extremely promising nanoplatform in cancer theranostics for targeted PTT under FL and PA dual-modal imaging.
靶向光疗和多模态成像可有效提高诊疗效果并减少其副作用。在此，我们构建了新型生物相容性花菁染料IR808偶联透明质酸纳米颗粒（HAIR NPs），用于近红外荧光（FL）和光声（PA）双模态成像的光热治疗（PTT）。纳米颗粒在水性条件下形成稳定的纳米结构，尺寸分布均匀。HAIR NPs通过CD44（肿瘤细胞表面的透明质酸受体）受体介导的内吞作用被人肺癌细胞A549快速摄取。激光照射后，HAIR NPs在荷瘤小鼠中实现了良好的近红外荧光成像和光声成像。此外，HA与IR808之间的共价连接产生的紧密纳米结构可显著提高IR808的光热转换效率。在低剂量激光功率下，HAIR NPs在体外和体内对肿瘤生长的抑制作用比游离IR808表现出更有效的光热治疗效果。总体而言，这些结果表明，HAIR NPs可能是用于FL和PA双模态成像下靶向PTT的癌症诊疗中极具前景的纳米平台。
