在连续流海绵生物反应器中培养反硝化厌氧甲烷氧化微生物。
Cultivation of denitrifying anaerobic methane-oxidizing microorganisms in a continuous-flow sponge bioreactor.
作者信息
Hatamoto Masashi, Sato Takafumi, Nemoto Sho, Yamaguchi Takashi
机构信息
Department of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
Top Runner Incubation Center for Academia-Industry Fusion, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.
出版信息
Appl Microbiol Biotechnol. 2017 Jul;101(14):5881-5888. doi: 10.1007/s00253-017-8315-4. Epub 2017 May 8.
Anaerobic treatment of sewage has many advantages; however, the effluent contains high levels of dissolved methane. In this study, we investigated the use of a closed-type downflow hanging sponge (DHS) reactor for application of the denitrifying anaerobic methane oxidation (DAMO) reaction for nitrogen and dissolved methane removal. When using nitrate, the DAMO reaction achieved a denitrification rate of 84.4 g N m day, which is close to that required for practical application of denitrification to anaerobic sewage treatment. The microbial community that developed in the DHS was investigated using16S rRNA, and novel species of DAMO bacteria affiliated with Group b of NC10 phylum were enriched. This contrasted with the results of previous studies in which the Candidatus Methylomirabilis oxyfera affiliated with Group a was enriched. The results obtained herein suggest that a post-treatment system for anaerobically treated sewage using a closed-type DHS reactor may become practical in the near future.
污水的厌氧处理有许多优点；然而，其流出物含有高浓度的溶解甲烷。在本研究中，我们研究了使用封闭式下流式悬挂海绵（DHS）反应器应用反硝化厌氧甲烷氧化（DAMO）反应来去除氮和溶解甲烷。使用硝酸盐时，DAMO反应实现了84.4 g N m⁻³ d⁻¹的反硝化速率，这接近反硝化在厌氧污水处理实际应用所需的速率。使用16S rRNA对DHS中形成的微生物群落进行了研究，隶属于NC10门b组的新型DAMO细菌物种得到了富集。这与之前研究中隶属于a组的“食甲基菌候选菌”得到富集的结果形成了对比。本文获得的结果表明，使用封闭式DHS反应器的厌氧处理污水后处理系统可能在不久的将来成为现实。
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