College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.
Microb Biotechnol. 2021 Jul;14(4):1397-1408. doi: 10.1111/1751-7915.13726. Epub 2020 Dec 11.
After the occurrence of nitrate-dependent anaerobic methane oxidation (AMO) in rumen fluid culture was proved, the organisms that perform the denitrifying anaerobic methane oxidizing (DAMO) process in the rumen of dairy goat were investigated by establishing two enrichment culture systems, which were supplied with methane as the sole carbon source and NaNO or NaNO as the electron acceptor. Several Operational Taxonomic Units (OTU) belonging to Proteobacteria became dominant in the two enrichment systems. The identified Pseudomonas aeruginosa, which was isolated from the NaNO enrichment system, could individually perform a whole denitrifying anaerobic methane oxidizing process. Further in vitro rumen fermentation showed that supplementation with the isolated P. aeruginosa could reduce methane emissions, alleviate the nitrite accumulation and prevent the decrease in propionic acid product caused by nitrate supplementation.
在证明反刍动物瘤胃液中存在依赖硝酸盐的厌氧甲烷氧化（AMO）之后，通过建立两个富集培养系统，以甲烷作为唯一碳源，以 NaNO3或 NaNO2作为电子受体，研究了在山羊瘤胃中进行反硝化厌氧甲烷氧化（DAMO）过程的微生物。两个富集系统中都有几个属于变形菌门的操作分类单元（OTU）成为优势菌。从 NaNO3富集系统中分离出的铜绿假单胞菌（Pseudomonas aeruginosa）能够单独完成整个反硝化厌氧甲烷氧化过程。进一步的体外瘤胃发酵表明，添加分离出的铜绿假单胞菌可以减少甲烷排放，缓解亚硝酸盐积累，并防止硝酸盐添加导致丙酸产量下降。
