果生链核盘菌α-L-阿拉伯呋喃糖苷酶III的单质子催化作用。
One-proton catalysis by the alpha-L-arabinofuranosidase III of Monilinia fructigena.
作者信息
Selwood T, Sinnott M L
机构信息
Department of Organic Chemistry, University of Bristol, U.K.
出版信息
Biochem J. 1988 Sep 15;254(3):899-901. doi: 10.1042/bj2540899.
Abstract
- Michaelis-Menten parameters for the hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside were measured as a function of pL (pH or pD) in both 1H2O and 2H2O. 2. The variation of both Vmax. and Vmax./Km with pL is sigmoid, the pK governing Vmax. shifting from 6.34 +/- 0.05 in 1H2O to 6.84 +/- 0.07 in 2H2O, and that governing Vmax./Km from 5.89 +/- 0.03 in 1H2O to 6.38 +/- 0.05 in 2H2O. 3. In the plateau regions there is a small inverse solvent isotope effect on Vmax./Km (0.92), and one of 1.45 on Vmax. 4. The variation of Vmax. with isotopic composition is strictly linear, indicating that the isotope effect arises from the transfer of a single proton.
摘要
- 对对硝基苯基α-L-阿拉伯呋喃糖苷水解反应的米氏参数，在H₂O和D₂O中均作为pL（pH或pD）的函数进行了测定。2. Vmax以及Vmax/Km随pL的变化呈S形，控制Vmax的pK值从H₂O中的6.34±0.05移至D₂O中的6.84±0.07，而控制Vmax/Km的pK值从H₂O中的5.89±0.03移至D₂O中的6.38±0.05。3. 在平稳区域，对Vmax/Km存在较小的反向溶剂同位素效应（0.92），对Vmax存在1.45的效应。4. Vmax随同位素组成的变化严格呈线性，表明同位素效应源于单个质子的转移。
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