Venkatasubban K S, Schowen R L
CRC Crit Rev Biochem. 1984;17(1):1-44. doi: 10.3109/10409238409110268.
The proton inventory technique uses the dependence of enzymic reaction rate on the atom fraction of deuterium present in mixtures of protium oxide and deuterium oxide to deduce for simple cases the number of exchangeable hydrogenic sites that produce isotope effects, and the magnitude of the isotope effect generated at each site. For more complex cases, other information, such as the participation of more than a single step in limiting the rate, may be obtained. The background of the method, the conduct of the experiments and the interpretation of the results are briefly reviewed. The method is then illustrated in its application to various enzyme systems by a series of case histories.
质子存量技术利用酶促反应速率对氧化氢和氧化氘混合物中氘的原子分数的依赖性，在简单情况下推断产生同位素效应的可交换氢位点的数量，以及每个位点产生的同位素效应的大小。对于更复杂的情况，可以获得其他信息，例如在限制速率方面不止一个步骤的参与情况。本文简要回顾了该方法的背景、实验操作和结果解释。然后通过一系列实例展示了该方法在各种酶系统中的应用。
