The Key Laboratory of Rare Earth Functional Materials and Applications, Zhoukou Normal University , Zhoukou 466001, P. R. China.
School of Mechanical & Aerospace Engineering, Nanyang Technological University , 50 Nanyang Avenue, 639798 Singapore.
ACS Appl Mater Interfaces. 2017 May 31;9(21):17718-17726. doi: 10.1021/acsami.7b02126. Epub 2017 May 16.
Three-dimensional elastic nanofibers (3D eNFs) can offer a suitable 3D dynamic microenvironment and sufficient flexibility to regulate cellular behavior and functional protein expression. In this study, we report a novel approach to prepare 3D nanofibers with excellent mechanical properties by solution-assisted electrospinning technology and in situ polymerization. The obtained 3D eNFs demonstrated excellent biocompatible properties to meet cell culture requirements under a dynamic environment in vitro. Moreover, these 3D eNFs also promoted human bone marrow mesenchymal stem cells (hMSCs) adhesion and collagen expression under biomechanical stimulation. The results demonstrated that this dynamic cell culture system could positively impact cellular collagen but has no significant effect on the proliferation of hMSCs grown in the 3D eNFs. This work may give rise to a new approach for constructing a 3D cell culture for tissue engineering.
三维弹性纳米纤维（3D eNFs）可为细胞行为和功能蛋白表达提供适宜的三维动态微环境和足够的灵活性。在本研究中，我们报告了一种通过溶液辅助静电纺丝技术和原位聚合制备具有优异机械性能的 3D 纳米纤维的新方法。所得到的 3D eNFs 在体外动态环境下表现出优异的生物相容性，满足细胞培养的要求。此外，这些 3D eNFs 还在生物力学刺激下促进人骨髓间充质干细胞（hMSCs）的黏附和胶原蛋白的表达。结果表明，这种动态细胞培养系统可以积极影响细胞胶原蛋白，但对 3D eNFs 中生长的 hMSCs 的增殖没有显著影响。这项工作可能为组织工程构建 3D 细胞培养提供一种新方法。
