Jin Lin, Xu Qinwei, Wu Shuyi, Kuddannaya Shreyas, Li Cheng, Huang Jingbin, Zhang Yilei, Wang Zhenling
The Key Laboratory of Rare Earth Functional Materials and Applications, Zhoukou Normal University, Zhoukou 466001, P. R. China.
School of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
ACS Biomater Sci Eng. 2016 Nov 14;2(11):2042-2049. doi: 10.1021/acsbiomaterials.6b00455. Epub 2016 Sep 21.
In recent years, three-dimensional (3D) scaffolds have proven to be highly advantageous in mammalian cell culture and tissue engineering compared to 2D substrates. Herein, we demonstrated the fabrication of novel 3D core-shell nanofibers (3D-CSNFs) using an improved electrospinning process combined with in situ surface polymerization. The obtained 3D nanofibrous scaffold displayed excellent mechanical and electrical properties. Moreover, the cotton-like 3D structure with large internal connected pores (20-100 μm) enabled cells to easily infiltrate into the interior of the 3D scaffold with a good spatial distribution to mimic the ECM-like cell microenvironments. Stable cell-fiber composite constructs were formed in the 3D-CSNFs with relatively higher adhesion and viability compared to 2D-CSNFs. Furthermore, the human mesenchymal stem cells (hMSCs) cultured on conductive polymer coated electrically active 3D nanofibers responded with a healthy morphology and anchorage on the fibers with relatively higher viability and proliferation under electrical stimulation (ES). This study demonstrates the successful fabrication of 3D-CSNFs and the constructive interaction of the 3D microenvironment and subsequent electrical stimulations on hMSCs, thereby holding promising potential in tissue engineering and regenerative therapies aided by electro-stimulation-based differentiation strategies.
近年来，与二维基质相比，三维（3D）支架已被证明在哺乳动物细胞培养和组织工程中具有高度优势。在此，我们展示了使用改进的静电纺丝工艺结合原位表面聚合制备新型三维核壳纳米纤维（3D-CSNFs）。所获得的三维纳米纤维支架表现出优异的机械和电学性能。此外，具有大的内部连通孔（20-100μm）的棉状三维结构使细胞能够轻松渗透到三维支架内部，并具有良好的空间分布，以模拟类似细胞外基质的细胞微环境。与二维CSNFs相比，在3D-CSNFs中形成了具有相对较高粘附力和活力的稳定细胞-纤维复合结构。此外，在导电聚合物涂层的电活性三维纳米纤维上培养的人间充质干细胞（hMSCs）呈现出健康的形态，并在纤维上锚定，在电刺激（ES）下具有相对较高的活力和增殖能力。本研究证明了3D-CSNFs的成功制备以及三维微环境与随后的电刺激对hMSCs的建设性相互作用，从而在基于电刺激分化策略的组织工程和再生治疗中具有广阔的应用前景。
