Sato Keigo, Mera Hisashi, Wakitani Shigeyuki, Takagi Mutsumi
a Division of Biotechnology and Macromolecular Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
b School of Health and Sports Sciences , Mukogawa Women's University , Nishinomiya , Japan.
Biosci Biotechnol Biochem. 2017 Jun;81(6):1241-1245. doi: 10.1080/09168451.2017.1282809. Epub 2017 Feb 28.
With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen. On the other hand, the addition of EGCG markedly decreased both the matrix metalloproteinase-13 concentration in the supernatant of cell sheet culture and the type II collagen degradation activity in that supernatant. Taken together, EGCG may enhance the accumulation of type II collagen by suppressing type II collagen degradation.
为了增加使用人骨髓间充质干细胞的无支架软骨样细胞片中II型胶原蛋白的含量，我们研究了在用于细胞片培养的软骨形成培养基中添加表没食子儿茶素-3-没食子酸酯（EGCG）的效果。添加EGCG（10 μM）使II型胶原蛋白的含量增加了2倍，而该添加并未显著改变编码II型胶原蛋白和Sox 9的基因的表达水平。细胞片中细胞内的活性氧水平被认为过低，无法抑制II型胶原蛋白的积累。另一方面，添加EGCG显著降低了细胞片培养上清液中基质金属蛋白酶-13的浓度以及该上清液中II型胶原蛋白的降解活性。综上所述，EGCG可能通过抑制II型胶原蛋白的降解来增强其积累。
