Ueno Mikinori, Nishiguchi Tomoki, Takeshita Satoshi, Yamaguchi Kenichi, Oda Tatsuya
a Graduate School of Fisheries Science and Environmental Studies , Nagasaki University , Nagasaki , Japan.
b Joint Research Division , Center for Industry, University and Government Corporation, Nagasaki University , Nagasaki , Japan.
Biosci Biotechnol Biochem. 2017 Jun;81(6):1254-1260. doi: 10.1080/09168451.2017.1292836. Epub 2017 Feb 20.
Enzymatically prepared alginate oligomer (AO) promoted the growth of Chlamydomonas reinhardtii in a concentration-dependent manner. AO at 2.5 mg/mL induced increase in expression levels of cyclin A, cyclin B, and cyclin D in C. reinhardtii. CuSO at 100 μM suppressed the growth of C. reinhardtiin, and AO at 2.5 mg/mL significantly alleviated the toxicity of CuSO. Increased intracellular reactive oxygen species level in C. reinhardtii induced by CuSO was reduced by AO. After cultivation with CuSO at 100 μM, expression levels of ascorbate peroxidase and superoxide dismutase in C. reinhardtii were increased, and AO reduced the increased levels of these enzymes. These results suggest that AO exhibits beneficial effects on C. reinhardtii through influencing the expression of various genes not only at normal growth condition but also under CuSO stress.
酶法制备的海藻酸钠寡聚物（AO）以浓度依赖的方式促进莱茵衣藻的生长。2.5毫克/毫升的AO诱导莱茵衣藻中细胞周期蛋白A、细胞周期蛋白B和细胞周期蛋白D的表达水平增加。100微摩尔的硫酸铜抑制莱茵衣藻的生长，而2.5毫克/毫升的AO显著减轻了硫酸铜的毒性。AO降低了硫酸铜诱导的莱茵衣藻细胞内活性氧水平的升高。在用100微摩尔硫酸铜培养后，莱茵衣藻中抗坏血酸过氧化物酶和超氧化物歧化酶的表达水平升高，而AO降低了这些酶升高的水平。这些结果表明，AO不仅在正常生长条件下，而且在硫酸铜胁迫下，通过影响各种基因的表达对莱茵衣藻表现出有益作用。
