Laboratory of Environmental Biology, Department of Applied Aquabiology, National Fisheries University, 2-7-1 Nagata-Honmachi, Shimonoseki, Yamaguchi 759-6595, Japan.
J Biosci Bioeng. 2012 Jan;113(1):112-6. doi: 10.1016/j.jbiosc.2011.09.009. Epub 2011 Oct 20.
Alginate is a natural acidic linear polysaccharide that is produced by brown seaweeds. It is currently used in a broad range of commercial enterprises, such as the food and medical products industries. Recent evidence has demonstrated that alginate oligosaccharides may function as growth promoting agents for certain plant cells, including those of some green algae. Chlamydomonas reinhardtii is a green alga that is used as a model organism in fundamental molecular biology studies; it is also a producer of biohydrogen. In the present study, we examined effects of two types of alginate oligosaccharide mixtures (AOMs), which were prepared by either enzymatic degradation (ED) or acid hydrolysis (AH), on the growth of C. reinhardtii. Growth was significantly promoted by AOM (ED) in a concentration-dependent manner. The maximum effect was observed on day 4 of treatment. The fatty acid composition of C. reinhardtii was also influenced by AOM (ED); the levels of C16:0, C18:2 cis and C18:3 n-3 increased in treated cells. AOM (AH) and the other saccharides that we tested did not affect the growth of C. reinhardtii. The effects that we identified could promote efficient biomass production by reducing culture times and by changing cellular fatty acid levels.
藻酸盐是一种天然酸性线性多糖，由褐藻产生。它目前广泛应用于各种商业企业，如食品和医疗产品行业。最近的证据表明，藻酸盐寡糖可能作为某些植物细胞（包括某些绿藻细胞）的生长促进剂发挥作用。莱茵衣藻是一种绿藻，被用作基础分子生物学研究的模式生物；它也是生物氢的生产者。在本研究中，我们研究了两种藻酸盐寡糖混合物（AOM）对莱茵衣藻生长的影响，这两种混合物是通过酶解（ED）或酸水解（AH）制备的。AOM（ED）以浓度依赖的方式显著促进了生长。在处理的第 4 天观察到最大效果。AOM（ED）还影响了莱茵衣藻的脂肪酸组成；处理细胞中的 C16:0、C18:2 cis 和 C18:3 n-3 水平增加。AOM（AH）和我们测试的其他糖没有影响莱茵衣藻的生长。我们发现的这些影响可以通过缩短培养时间和改变细胞脂肪酸水平来促进有效的生物质生产。
