Smyth Megan, Fournier Carole, Driemeier Carlos, Picart Catherine, Foster E Johan, Bras Julien
CNRS, LGP2 , 461 Rue de la Papeterie, 38402, Saint-Martin-d'Hères, France.
Université Grenoble Alpes, LGP2 , 38000 Grenoble, France.
Biomacromolecules. 2017 Jul 10;18(7):2034-2044. doi: 10.1021/acs.biomac.7b00209. Epub 2017 Jun 28.
Thin cellulose nanofiber (CNF) nanostructured substrates with varying roughness, stiffness (Young's modulus), porosity, and swelling properties were produced by varying the conditions used during fabrication. It was shown that with increased heat exposure, CNF substrate porosity in an aqueous state decreased while Young's modulus in a water submerged state increased. In this study, the adhesion and viability of mesenchymal stem cells (MSCs) cultured on this CNF substrate will be presented. Viability of D1/BALBc MSCs were assessed for 24 and 48 h, and it was shown that depending on the CNF substrate the viability varied significantly. The adhesion of MSCs after 6 and 24 h was conditional on material mechanical properties and porosity of the CNF in cell culture conditions. These results suggest that material properties of CNF nanostructured substrate within the aqueous state can be easily tuned with curing step without any chemical modification to the CNF and that these changes can affect MSC viability in cell culture.
通过改变制备过程中使用的条件，制备出了具有不同粗糙度、刚度（杨氏模量）、孔隙率和溶胀特性的薄纤维素纳米纤维（CNF）纳米结构基底。结果表明，随着热暴露增加，处于水合状态的CNF基底孔隙率降低，而处于水浸状态的杨氏模量增加。在本研究中，将展示在这种CNF基底上培养的间充质干细胞（MSC）的黏附情况和活力。对D1/BALBc MSCs的活力进行了24小时和48小时的评估，结果表明，根据CNF基底的不同，活力有显著差异。在细胞培养条件下，MSC在6小时和24小时后的黏附情况取决于材料的力学性能和CNF的孔隙率。这些结果表明，在水合状态下，CNF纳米结构基底的材料性能可以通过固化步骤轻松调节，而无需对CNF进行任何化学修饰，并且这些变化会影响细胞培养中MSC的活力。
