Kim Jeongjin, Ahn Juhee
Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology, Kangwon National University , Chuncheon, Republic of Korea.
Microb Drug Resist. 2017 Dec;23(8):949-957. doi: 10.1089/mdr.2016.0319. Epub 2017 May 9.
This study was designed mainly to assess the phenotypic properties of clinically isolated Salmonella Typhimurium exposed to ceftriaxone and ciprofloxacin. The antibiotic susceptibility, β-lactamase activity, efflux activity, bacterial motility, biofilm-forming ability, and gene expression were determined in S. Typhimurium ATCC 19585 and S. Typhimurium CCARM 8009 when exposed to subinhibitory concentrations of ceftriaxone and ciprofloxacin. S. Typhimurium CCARM 8009 was highly resistant to ampicillin, kanamycin, penicillin G, and streptomycin, showing minimum inhibitory concentration values of more than 512 μg/ml, while S. Typhimurium ATCC 19585 showed resistance to erythromycin alone (64 μg/ml). The highest β-lactamase activity was observed in S. Typhimurium CCARM 8009 when exposed to ceftriaxone (8.2 μmol/min/ml), while the least β-lactamase activity was observed in S. Typhimurium ATCC 19585. Compared to S. Typhimurium CCARM 8009, the ethidium bromide (EtBr) accumulation was considerably increased in S. Typhimurium ATCC 19585 when treated with efflux pump inhibitors. S. Typhimurium ATCC 19585 and S. Typhimurium CCARM 8009 were highly susceptible to ciprofloxacin, erythromycin, levofloxacin, and sparfloxacin in the presence of phenylalanine-arginine-β-naphthylamide. The swimming motility of S. Typhimurium ATCC 19585 exposed to ceftriaxone was significantly reduced to 54% when compared to S. Typhimurium CCARM 8009 (93%). The numbers of attached S. Typhimurium CCARM 8009 cells were significantly increased by more than 1 log cfu/ml when exposed to ceftriaxone and ciprofloxacin. The relative gene expression was stable in S. Typhimurium CCARM 8009 in the presence of ceftriaxone and ciprofloxacin compared to the absence of antibiotics. These results suggest that the antibiotic susceptibility of S. Typhimurium having different antibiotic resistance profiles varied depending on the presence of ceftriaxone and ciprofloxacin.
本研究主要旨在评估临床分离的鼠伤寒沙门氏菌在接触头孢曲松和环丙沙星后的表型特性。测定了鼠伤寒沙门氏菌ATCC 19585和鼠伤寒沙门氏菌CCARM 8009在接触亚抑菌浓度的头孢曲松和环丙沙星时的抗生素敏感性、β-内酰胺酶活性、外排活性、细菌运动性、生物膜形成能力及基因表达。鼠伤寒沙门氏菌CCARM 8009对氨苄西林、卡那霉素、青霉素G和链霉素高度耐药，最低抑菌浓度值超过512μg/ml，而鼠伤寒沙门氏菌ATCC 19585仅对红霉素耐药（64μg/ml）。接触头孢曲松时，鼠伤寒沙门氏菌CCARM 8009的β-内酰胺酶活性最高（8.2μmol/min/ml），而鼠伤寒沙门氏菌ATCC 19585的β-内酰胺酶活性最低。与鼠伤寒沙门氏菌CCARM 8009相比，用外排泵抑制剂处理时，鼠伤寒沙门氏菌ATCC 19585中溴化乙锭（EtBr）的积累显著增加。在苯丙氨酸-精氨酸-β-萘酰胺存在的情况下，鼠伤寒沙门氏菌ATCC 19585和鼠伤寒沙门氏菌CCARM 8009对环丙沙星、红霉素、左氧氟沙星和司帕沙星高度敏感。与鼠伤寒沙门氏菌CCARM 8009（93%）相比，接触头孢曲松的鼠伤寒沙门氏菌ATCC 19585的游动运动性显著降低至54%。接触头孢曲松和环丙沙星时，附着的鼠伤寒沙门氏菌CCARM 8009细胞数量显著增加，超过1 log cfu/ml。与不存在抗生素相比，在头孢曲松和环丙沙星存在的情况下，鼠伤寒沙门氏菌CCARM 8009中的相对基因表达稳定。这些结果表明，具有不同抗生素耐药谱的鼠伤寒沙门氏菌的抗生素敏感性因头孢曲松和环丙沙星的存在而有所不同。
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