Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 24341, Republic of Korea.
Arch Microbiol. 2019 Nov;201(9):1259-1275. doi: 10.1007/s00203-019-01693-1. Epub 2019 Jun 25.
This study was designed to compare the differentially expressed proteins between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium, Klebsiella pneumonia, and Staphylococcus aureus. The susceptibilities of wild-type (WT), ciprofloxacin (CIP) and/or oxacillin (OXA)-induced, and clinically isolated resistant (CCARM) S. Typhimurium (ST, ST, and ST), K. pneumoniae (KP, KP, and KP), and S. aureus (SA, SA, SA, and SA) to antibiotics were determined using broth microdilution assay. ST was highly resistant to piperacillin (MIC > 512 μg/ml), KP was resistant to chloramphenicol (128 μg/ml) and norfloxacin (16 μg/ml), SA was resistant to fluoroquinolones (32 μg/ml), and SA was resistant to ceftriaxone (32 μg/ml). The protein profiles of antibiotic-sensitive and antibiotic-resistant strains were determined using 2-DE analysis followed by LC-MS/MS. The commonly expressed proteins of ST-ST, ST-ST, KP-KP, KP-KP, SA-SA, SA-SA, and SA-SA were 763, 677, 677, 469, 261, 259, and 226, respectively. The unique protein spots were observed 57 (6.5%), 80 (11.5%), and 68 (13.9%), respectively, for ST, KP, and SA. The highly up-regulated protein, PrsA (10-fold), was observed in ST resistant to ciprofloxacin (128-fold), levofloxacin (32-fold), norfloxacin (64-fold), and piperacillin (> 16-fold). The up-regulated proteins (YadC, FimA, and RplB) in KP resistant to chloramphenicol (> 32-fold), ciprofloxacin (32-fold), levofloxacin (6-fold), norfloxacin (128-fold), and sparfloxacin (64-fold). AcrB and RpoB were up-regulated in SA resistant to multiple antibiotics. The differentially expressed proteins were related to the antibiotic resistance of ST, ST, ST, KP, KP, KP, SA, SA, SA, and SA. The resistance-associated proteins could be useful biomarkers for detecting antibiotic-resistant pathogens.
这项研究旨在比较抗生素敏感和耐药的鼠伤寒沙门氏菌、肺炎克雷伯菌和金黄色葡萄球菌之间的差异表达蛋白。使用肉汤微量稀释法测定野生型（WT）、环丙沙星（CIP）和/或苯唑西林（OXA）诱导的以及临床分离的耐药（CCARM）鼠伤寒沙门氏菌（ST、ST 和 ST）、肺炎克雷伯菌（KP、KP 和 KP）和金黄色葡萄球菌（SA、SA、SA 和 SA）对抗生素的敏感性。ST 对哌拉西林高度耐药（MIC>512μg/ml），KP 对氯霉素（128μg/ml）和诺氟沙星（16μg/ml）耐药，SA 对氟喹诺酮类药物（32μg/ml）耐药，SA 对头孢曲松（32μg/ml）耐药。使用 2-DE 分析结合 LC-MS/MS 测定抗生素敏感和耐药菌株的蛋白质图谱。ST-ST、ST-ST、KP-KP、KP-KP、SA-SA、SA-SA 和 SA-SA 的共同表达蛋白分别为 763、677、677、469、261、259 和 226。ST、KP 和 SA 分别观察到 57 个（6.5%）、80 个（11.5%）和 68 个（13.9%）独特的蛋白质斑点。对环丙沙星（128 倍）、左氧氟沙星（32 倍）、诺氟沙星（64 倍）和哌拉西林（>16 倍）耐药的 ciprofloxacin 的 ST 中观察到高度上调的蛋白 PrsA（10 倍）。对氯霉素（>32 倍）、环丙沙星（32 倍）、左氧氟沙星（6 倍）、诺氟沙星（128 倍）和 sparfloxacin（64 倍）耐药的 KP 中上调的蛋白（YadC、FimA 和 RplB）。AcrB 和 RpoB 在耐多种抗生素的 SA 中上调。差异表达的蛋白与 ST、ST、ST、KP、KP、KP、SA、SA、SA 和 SA 的抗生素耐药性有关。耐药相关蛋白可作为检测抗生素耐药病原体的有用生物标志物。
