Chien Jason L, Ghassibi Mark P, Mahadeshwar Pooja, Li Pengcheng, Liebmann Jeffrey M, Ritch Robert, Milman Tatyana, Park Sung Chul
*Moise and Chella Safra Advanced Ocular Imaging Laboratory, Einhorn Clinical Research Center, New York Eye and Ear Infirmary of Mount Sinai §Department of Pathology and Laboratory Medicine, New York Eye and Ear Infirmary of Mount Sinai ‡Bernard and Shirlee Brown Glaucoma Research Laboratory, Harkness Eye Institute, Columbia University Medical Center, Harkness Eye Institute ∥Department of Ophthalmology, Manhattan Eye, Ear and Throat Hospital, New York ¶Department of Ophthalmology, Hofstra Northwell School of Medicine, Hempstead, NY †George Washington University School of Medicine and Health Sciences, Washington, DC.
J Glaucoma. 2017 Jul;26(7):626-632. doi: 10.1097/IJG.0000000000000685.
The purpose of this study is to investigate the use of anterior segment enhanced depth imaging (EDI) optical coherence tomography (OCT) for ex vivo lamina cribrosa (LC) imaging.
After removing anterior segment and vitreous, the optic nerve head (ONH) tissue of porcine eyes was placed on a customized eye holder for imaging. Serial EDI OCT B-scans (interval, ∼35 μm) of the ONH were obtained using anterior segment module of spectral-domain OCT. Various conditions were tested for better quality LC images. After EDI OCT, serial histologic sections were obtained (distance between sections, ∼5 μm). LC structures in OCT scans were compared with those in histologic sections. Three-dimensional LC reconstructions created using serial OCT scans were compared with LC structures in disc photographs.
ONHs of 3 enucleated eyes were examined. The LC was more clearly imaged when the retina and part of the prelaminar tissue were removed (quality score, 39.01±3.30 vs. 26.40±5.85; P<0.001) and when the tissue was kept moist during imaging (quality score, 38.70±2.11 vs. 36.18±5.98; P<0.001). LC image quality was similar before and after fixation (quality score, 38.84±6.57 vs. 39.21±9.69; P=0.79). LC beams and part of retrolaminar glial columns identified in OCT scans matched those in histologic sections. LC beams and pores in 3-dimensional reconstructions matched those in disc photographs.
High-resolution cross-sectional images of the LC, comparable to histologic sections, can be obtained using anterior segment EDI OCT in ex vivo eyes with proper tissue preparation.
本研究旨在探讨前段增强深度成像（EDI）光学相干断层扫描（OCT）用于离体筛板（LC）成像的情况。
去除前段和玻璃体后，将猪眼的视神经乳头（ONH）组织置于定制的眼托上进行成像。使用光谱域OCT的前段模块获取ONH的系列EDI OCT B扫描图像（间隔约35μm）。测试了各种条件以获取质量更好的LC图像。EDI OCT检查后，获取系列组织学切片（切片间距约5μm）。将OCT扫描中的LC结构与组织学切片中的结构进行比较。将使用系列OCT扫描创建的三维LC重建与视盘照片中的LC结构进行比较。
检查了3只摘除眼球的ONH。当去除视网膜和部分板前组织时（质量评分，39.01±3.30对26.40±5.85；P<0.001）以及成像过程中组织保持湿润时（质量评分，38.70±2.11对36.18±5.98；P<0.001），LC成像更清晰。固定前后LC图像质量相似（质量评分，38.84±6.57对39.21±9.69；P=0.79）。OCT扫描中识别出的LC束和部分板后视神经胶质柱与组织学切片中的相符。三维重建中的LC束和孔隙与视盘照片中的相符。
通过前段EDI OCT并进行适当的组织制备，可在离体眼中获得与组织学切片相当的LC高分辨率横断面图像。
