Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, Austria.
Austrian Cluster for Tissue Regeneration, Vienna, Austria.
J Tissue Eng Regen Med. 2018 Feb;12(2):416-426. doi: 10.1002/term.2467. Epub 2017 Oct 20.
One of the mainstays of facial rejuvenation strategies is volume restoration, which can be achieved by autologous fat grafting. In our novel approach, we treated the adipose tissue harvest site with extracorporeal shock wave therapy (ESWT) in order to improve the quality of the regenerative cells in situ. The latter was demonstrated by characterizing the cells of the stromal vascular fraction (SVF) in the harvested liposuction material regarding cell yield, adenosine triphosphate (ATP) content, proliferative capacity, surface marker profile, differentiation potential and secretory protein profile. Although the SVF cell yield was only slightly enhanced, viability and ATP concentration of freshly isolated cells as well as proliferation doublings after 3 weeks in culture were significantly increased in the ESWT compared with the untreated group. Likewise, cells expressing mesenchymal and endothelial/pericytic markers were significantly elevated concomitant with an improved differentiation capacity towards the adipogenic lineage and enhancement in specific angiogenic proteins. Hence, in situ ESWT might be applied in the future to promote cell fitness, adipogenesis and angiogenesis within the fat graft for successful facial rejuvenation strategies with potential long-term graft survival.
面部年轻化策略的主要支柱之一是体积恢复，可以通过自体脂肪移植来实现。在我们的新方法中，我们通过体外冲击波治疗（ESWT）来处理脂肪组织采集部位，以改善原位再生细胞的质量。通过对采集的脂肪抽吸材料中的基质血管部分（SVF）细胞进行细胞产量、三磷酸腺苷（ATP）含量、增殖能力、表面标志物谱、分化潜能和分泌蛋白谱的特征化，证明了这一点。尽管 SVF 细胞产量仅略有增加，但与未处理组相比，新鲜分离细胞的活力和 ATP 浓度以及培养 3 周后的增殖倍数在 ESWT 组中显著增加。同样，表达间充质和内皮/周细胞标志物的细胞明显升高，同时向脂肪生成谱系的分化能力得到改善，并增强了特定的血管生成蛋白。因此，未来可能会在体内应用 ESWT 来促进脂肪移植中的细胞适应性、脂肪生成和血管生成，从而实现成功的面部年轻化策略，并具有潜在的长期移植物存活率。
