Chen Hengxi, Song Yong, Yang Shiyuan, Fu Jing, Feng Xue, Huang Wei
Department of Obstetrics and Gynecology, West China Second University Hospital of Sichuan University, Chengdu, People's Republic of China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital of Sichuan University, Chengdu, People's Republic of China.
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, People's Republic of China.
Placenta. 2017 May;53:30-35. doi: 10.1016/j.placenta.2017.03.013. Epub 2017 Mar 21.
The decidualization of uterine endometrial stromal cells (ESCs) is critical for the successful establishment and maintenance of pregnancy and involves extensive cell proliferation and differentiation. A newly established signaling pathway, the Hippo/Yes-associated protein (YAP) pathway, plays a critical role in these proliferation processes. Our previous study demonstrated that YAP is expressed in human ESCs. However, its role in decidualization remains unclear. The objective of the present study was to explore the role of YAP in the decidualization of human ESCs.
The expression of YAP was first investigated in the endometrium of non-pregnant women and in the decidua of pregnant women. The role of YAP was investigated by transfecting ESCs with mRNA silencing constructs and observing the negative effects of this action upon decidualization induced in vitro.
Our results revealed that the expression of YAP was higher in decidual cells from early pregnant decidua compared with ESCs from non-pregnant endometrium. The expression levels of YAP and TEA domain 1 (TEAD1) were both increased in ESCs during in vitro decidualization and the knockdown of YAP in ESCs caused negative effects upon decidualization in vitro.
Our study suggests that YAP is upregulated in human decidual cells compared with ESCs and influences the decidualization of ESCs.
子宫内膜基质细胞（ESCs）的蜕膜化对于成功建立和维持妊娠至关重要，且涉及广泛的细胞增殖和分化。一条新建立的信号通路，即Hippo/Yes相关蛋白（YAP）通路，在这些增殖过程中起关键作用。我们之前的研究表明YAP在人ESCs中表达。然而，其在蜕膜化中的作用仍不清楚。本研究的目的是探讨YAP在人ESCs蜕膜化中的作用。
首先研究YAP在非妊娠妇女子宫内膜和妊娠妇女蜕膜中的表达。通过用mRNA沉默构建体转染ESCs并观察此操作对体外诱导的蜕膜化的负面影响来研究YAP的作用。
我们的结果显示，与非妊娠子宫内膜的ESCs相比，早孕蜕膜的蜕膜细胞中YAP的表达更高。在体外蜕膜化过程中，ESCs中YAP和TEA结构域1（TEAD1）的表达水平均升高，并且ESCs中YAP的敲低对体外蜕膜化产生负面影响。
我们的研究表明，与ESCs相比，YAP在人蜕膜细胞中上调，并影响ESCs的蜕膜化。
