Rashid Muhammad Ibrahim, Naz Anam, Ali Amjad, Andleeb Saadia
Department of Industrial Biotechnology, Atta ur Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Pakistan.
Department of Industrial Biotechnology, Atta ur Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology (NUST), Islamabad, Pakistan.
Genomics. 2017 Jul;109(3-4):274-283. doi: 10.1016/j.ygeno.2017.05.001. Epub 2017 May 6.
Pseudomonas aeruginosa is among top critical nosocomial infectious agents due to its persistent infections and tendency for acquiring drug resistance mechanisms. To date, there is no vaccine available for this pathogen. We attempted to exploit the genomic and proteomic information of P. aeruginosa though reverse-vaccinology approaches to unveil the prospective vaccine candidates. P. aeruginosa strain PAO1 genome was subjected to sequential prioritization approach following genomic, proteomics and structural analyses. Among, the predicted vaccine candidates: surface components of antibiotic efflux pumps (Q9HY88, PA2837), chaperone-usher pathway components (CupC2, CupB3), penicillin binding protein of bacterial cell wall (PBP1a/mrcA), extracellular component of Type 3 secretory system (PscC) and three uncharacterized secretory proteins (PA0629, PA2822, PA0978) were identified as potential candidates qualifying all the set criteria. These proteins were then analyzed for potential immunogenic surface exposed epitopes. These predicted epitopes may provide a basis for development of a reliable subunit vaccine against P. aeruginosa.
铜绿假单胞菌因其持续性感染以及获得耐药机制的倾向，位列最关键的医院感染病原体之中。迄今为止，尚无针对该病原体的疫苗。我们试图通过反向疫苗学方法利用铜绿假单胞菌的基因组和蛋白质组信息，以揭示潜在的疫苗候选物。对铜绿假单胞菌PAO1菌株的基因组进行了基因组学、蛋白质组学和结构分析后，采用了顺序优先排序法。在预测的疫苗候选物中，抗生素外排泵的表面成分（Q9HY88、PA2837）、伴侣-分泌途径成分（CupC2、CupB3）、细菌细胞壁的青霉素结合蛋白（PBP1a/mrcA）、3型分泌系统的细胞外成分（PscC）以及三种未鉴定的分泌蛋白（PA0629、PA2822、PA0978）被确定为符合所有既定标准的潜在候选物。然后对这些蛋白质进行潜在免疫原性表面暴露表位分析。这些预测的表位可为开发一种可靠的抗铜绿假单胞菌亚单位疫苗提供依据。
