粗糙脉孢菌中的环核苷酸磷酸二酯酶活性。通过免疫亲和色谱法进行纯化及特性鉴定。

Cyclic nucleotide phosphodiesterase activity in Neurospora crassa. Purification by immunoaffinity chromatography and characterization.

作者信息

Ulloa R M, Rubinstein C P, Molina y Vedia L, Torres H N, Téllez-Iñón M T

机构信息

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Buenos Aires, Argentina.

出版信息

FEBS Lett. 1988 Dec 5;241(1-2):219-22. doi: 10.1016/0014-5793(88)81065-2.

DOI:10.1016/0014-5793(88)81065-2

PMID:2848723

Abstract

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin.

摘要

通过其抑制酶活性的能力筛选出针对粗糙脉孢菌环核苷酸磷酸二酯酶（PDE I）的单克隆抗体。将与琼脂糖4B偶联的单克隆免疫球蛋白用于PDE I活性的亲和纯化。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，亲和纯化的PDE I组分显示出一条约41 kDa的单一多肽带。这条带在蛋白质免疫印迹中与上述单克隆免疫球蛋白发生反应。