Schofield Zoe, Reed Michelle Ac, Newsome Philip N, Adams David H, Günther Ulrich L, Lalor Patricia F
Zoe Schofield, Sci-Phy-4-Health, EPSRC Research and Training Centre in Physical Sciences for Health, College of Engineering and Physical Sciences, Birmingham B152TT, United Kingdom.
World J Gastroenterol. 2017 Apr 21;23(15):2685-2695. doi: 10.3748/wjg.v23.i15.2685.
To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue.
We have carried out pilot study using H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries.
Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach.
Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.
为了解人类肝脏疾病潜在的代谢变化，我们将核磁共振（NMR）代谢组学分析应用于人类肝脏组织。
我们开展了一项初步研究，使用氢核磁共振（H-NMR）从患有脂肪变性、非酒精性脂肪性肝炎（NASH）或酒精相关性肝损伤（ARLD）的患者的人类肝脏中获取代谢组学特征，以识别能够预测预后并区分酒精性和代谢性肝损伤的物质。
支链氨基酸稳态、三羧酸循环和嘌呤生物合成中间体以及甜菜碱的变化与酒精相关性肝损伤和非酒精性脂肪性肝病中肝硬化的发展有关。使用我们的方法还检测到了如丙二醇等物质以及尚未鉴定的成分，这些成分能够区分NASH和ARLD样本。
我们用于人类肝脏标本中多种分析物定量的高通量、非破坏性技术具有识别具有预后和诊断意义的生物标志物的潜力。
