Fu Xiangjun, Fang Jugao, Lian Meng, Zhong Qi, Ma Hongzhi, Feng Ling, Wang Ru, Wang Haizhou
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China.
Mol Med Rep. 2017 Jun;15(6):4266-4272. doi: 10.3892/mmr.2017.6547. Epub 2017 May 3.
The present study aimed to investigate the microRNA (miRNA) profile in human medullary thyroid carcinoma (MTC) tissue. The GSE40807 data profile was downloaded from the Gene Expression Omnibus database. Following preprocessing, differentially expressed microRNAs (DEMs) between MTC and healthy tissues were identified. Based on the obtained DEMs, transcription factor (TF)‑miRNA and miRNA‑target gene regulatory association pairs were predicted. Finally, functional enrichment analysis was performed on target genes of DEMs. Fifteen upregulated and 17 downregulated DEMs were identified. In the constructed TF‑miRNA regulatory network, hsa‑miR‑9‑5p was regulated by 9 TFs and hsa‑miR‑1 was regulated by 8 TFs. TFs of nuclear factor of κ light polypeptide gene enhancer in B‑cells 1 (NF‑κB1) and v‑myc avian myelocytomatosis viral oncogene homolog (MYC) regulated 4 and 3 DEMs, respectively. In the miRNA‑target gene regulatory network, hsa‑miR‑1, hsa‑miR‑9‑5p, hsa‑miR‑96‑5p and hsa‑miR‑590‑5p were most upregulated. The target genes of these 4 miRNAs were primarily enriched in the mitogen activated protein kinase (MAPK) signaling pathway. Therefore, MAPK signaling pathway may serve important roles in MTC progression. In conclusion, the DEMs hsa‑miR‑1 and hsa‑miR‑9‑5p, and TFs of NF‑κB1 and MYC may be used as biomarkers for the diagnosis and treatment of MTC.
本研究旨在调查人甲状腺髓样癌（MTC）组织中的微小RNA（miRNA）谱。从基因表达综合数据库下载GSE40807数据谱。经过预处理后，鉴定出MTC与健康组织之间差异表达的微小RNA（DEM）。基于获得的DEM，预测转录因子（TF）-miRNA和miRNA-靶基因调控关联对。最后，对DEM的靶基因进行功能富集分析。鉴定出15个上调的DEM和17个下调的DEM。在构建的TF-miRNA调控网络中，hsa-miR-9-5p受9个TF调控，hsa-miR-1受8个TF调控。B细胞κ轻链基因增强子核因子1（NF-κB1）和v-myc禽骨髓细胞瘤病毒癌基因同源物（MYC）的TF分别调控4个和3个DEM。在miRNA-靶基因调控网络中，hsa-miR-1、hsa-miR-9-5p、hsa-miR-96-5p和hsa-miR-590-5p上调最为明显。这4种miRNA的靶基因主要富集在丝裂原活化蛋白激酶（MAPK）信号通路中。因此，MAPK信号通路可能在MTC进展中起重要作用。总之，DEM hsa-miR-1和hsa-miR-9-5p以及NF-κB1和MYC的TF可能用作MTC诊断和治疗的生物标志物。
