Pourjafar Mona, Saidijam Massoud, Etemadi Katayoon, Najafi Rezvan
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamedan, Iran.
Department of Immunology, Hamadan University of Medical Sciences, Hamedan, Iran.
Biotechnol Lett. 2017 Aug;39(8):1263-1268. doi: 10.1007/s10529-017-2350-1. Epub 2017 May 9.
To investigate the effect of all-trans retinoic acid (ATRA) on caspase 3 activity, matrix metalloproteinase 2 (MMP-2), and MMP-9 expression and activity as well as in vitro rat bone marrow-derived mesenchymal stem cells (MSCs) migration.
The expression of the MMP-2/-9 was at least five times higher in ATRA-treated MSCs (P < 0.001), and MMP-2/-9 activity was enhanced with increasing doses compared to the control MSCs. The caspase three activity was attenuated by ATRA preconditioning. Scratch test showed that ATRA could promote the migration capacity of the MSCs compared to the untreated MSCs in a dose-dependent manner.
ATRA increases the in vitro migration capacity of the MSCs through stimulating the expression and activity of MMP-2/-9 and inhibiting caspase three enzyme activity.
研究全反式维甲酸（ATRA）对大鼠骨髓间充质干细胞（MSCs）半胱天冬酶3活性、基质金属蛋白酶2（MMP-2）和MMP-9表达及活性以及体外迁移的影响。
与对照组MSCs相比，经ATRA处理的MSCs中MMP-2/-9的表达至少高五倍（P < 0.001），且随着剂量增加，MMP-2/-9活性增强。ATRA预处理可减弱半胱天冬酶3活性。划痕试验表明，与未处理的MSCs相比，ATRA能以剂量依赖的方式促进MSCs的迁移能力。
ATRA通过刺激MMP-2/-9的表达和活性以及抑制半胱天冬酶3酶活性，增加MSCs的体外迁移能力。
