Younsi Faten, Mehdi Sameh, Aissi Oumayma, Rahali Najoua, Jaouadi Rym, Boussaid Mohamed, Messaoud Chokri
Laboratory of Nanobiotechnologies and Valorisation of Medicinal Phytoresources, Department of Biology, National Institute of Applied Science and Technology, University of Carthage, B.P. 676, 1080, Tunis Cedex, Tunisia.
Faculty of Sciences of Bizerte, University of Carthage, Jarzoun, 7021, Bizerte, Tunisia.
Chem Biodivers. 2017 Jul;14(7). doi: 10.1002/cbdv.201700017. Epub 2017 Jun 15.
The intraspecific variability of Artemisia herba-alba and A. campestris essential oils and the evaluation of their antioxidant and antiacetylcholinesterase activities were determined. Artemisia herba-alba essential oil was found rich in camphor (19.61%), α-thujone (19.40%), β-thujone (9.44%), chrysanthenone (9.26%), and trans-sabinyl acetate (8.43%). The major compounds of A. campestris essential oil were germacrene D (16.38%), β-pinene (16.33%), and limonene (9.17%). Significant variation in the essential oil composition was observed among populations of each species. The divergence between populations was attributed to the variation of some climatic factors such as altitude, annual rainfall, winter cold stress, summer precipitation, summer drought stress, evapotranspiration, and humidity. Artemisia herba-alba and A. campestris essential oils exhibited promising antioxidant and antiacetylcholinesterase activities. The level of activity varied significantly according to the species and the essential oil. The highest scavenging activity (IC = 0.14 mg/ml) and the uppermost capacity to prevent β-carotene bleaching (IC = 0.10 mg/ml) characterized A. campestris from population 6. A. campestris population 3 possessed the uppermost ability to reduce ferric ions (450.7 μmol Fe /g EO). The population 2 of A. campestris showed the strongest antiacetylcholinesterase activity (IC = 0.02 mg/ml). The variation of these activities between the essential oils was explained by their composition differences.
测定了白蒿和原野蒿精油的种内变异性，并对其抗氧化和抗乙酰胆碱酯酶活性进行了评估。发现白蒿精油富含樟脑（19.61%）、α-侧柏酮（19.40%）、β-侧柏酮（9.44%）、菊烯酮（9.26%）和反式乙酸桧酯（8.43%）。原野蒿精油的主要成分是吉马烯D（16.38%）、β-蒎烯（16.33%）和柠檬烯（9.17%）。在每个物种的种群之间观察到精油成分存在显著差异。种群之间的差异归因于一些气候因素的变化，如海拔、年降雨量、冬季寒冷胁迫、夏季降水量、夏季干旱胁迫、蒸散量和湿度。白蒿和原野蒿精油表现出有前景的抗氧化和抗乙酰胆碱酯酶活性。活性水平因物种和精油而异。来自6号种群的原野蒿具有最高的清除活性（IC = 0.14 mg/ml）和最强的防止β-胡萝卜素漂白的能力（IC = 0.10 mg/ml）。原野蒿3号种群具有最高的还原铁离子能力（450.7 μmol Fe /g EO）。原野蒿2号种群表现出最强的抗乙酰胆碱酯酶活性（IC = 0.02 mg/ml）。这些活性在精油之间的差异是由它们的成分差异所解释的。
