Henning Tara R, Morris Monica, Ellis Shanon, Kelley Kristen, Phillips Christi, Ritter Jana, Jones Tara, Nachamkin Eli, Chen Cheng Y, Hong Jaeyoung, Kang Joseph, Patton Dorothy, McNicholl Janet, Papp John, Kersh Ellen N
Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Total Solutions, Atlanta, GA, USA.
J Med Primatol. 2017 Oct;46(5):218-227. doi: 10.1111/jmp.12272. Epub 2017 May 10.
Rectal STI coinfection models enhance the understanding of rectal HIV transmission risk factors.
Rhesus macaques (n=9) were exposed to one of three rectal Chlamydia trachomatis (CT) challenges: C. trachomatis L (CT-L ); C. trachomatis serovar E (CT-E), followed by CT-L ; or CT-E, treatment/clearance, then CT-L . Infections were monitored by PCR. Weekly blood and rectal secretion/lavage samples were collected for cytokine analyzes and/or epithelial sloughing, occult, and overt blood determinations.
Chlamydial infections were successfully established in each animal, with varying degrees of persistence. Mucosal IL-1beta was upregulated in animals consecutively infected with CT-E then CT-L (P=.05). Epithelial sloughing was also significantly increased post-infection in this group (P=.0003).
This study demonstrates successful rectal infection of rhesus macaques with CT-E and CT-L and describes measures of assessing rectal inflammation and pathology. Different infection strategies yield varying inflammatory and pathologic outcomes, providing well-described models for future SIV/SHIV susceptibility studies.
直肠性传播感染合并感染模型有助于增强对直肠艾滋病毒传播风险因素的理解。
将9只恒河猴暴露于三种直肠沙眼衣原体（CT）感染挑战之一：沙眼衣原体L型（CT-L）；沙眼衣原体血清型E（CT-E），随后感染CT-L；或CT-E，治疗/清除后再感染CT-L。通过聚合酶链反应监测感染情况。每周采集血液和直肠分泌物/灌洗样本进行细胞因子分析和/或上皮脱落、潜血和显性血液测定。
每只动物均成功建立衣原体感染，且感染持续程度各异。在先后感染CT-E和CT-L的动物中，黏膜白细胞介素-1β上调（P = 0.05）。该组感染后上皮脱落也显著增加（P = 0.0003）。
本研究证明恒河猴可成功经直肠感染CT-E和CT-L，并描述了评估直肠炎症和病理的方法。不同的感染策略会产生不同的炎症和病理结果，为未来的猴免疫缺陷病毒/猿猴免疫缺陷病毒易感性研究提供了详细的模型。
