Díez Juana, Jungfleisch Jennifer
a Molecular Virology Group, Department of Experimental and Health Sciences , Universitat Pompeu Fabra , Barcelona , Spain.
RNA Biol. 2017 Jul 3;14(7):835-837. doi: 10.1080/15476286.2017.1325068. Epub 2017 May 10.
Viruses are powerful tools to uncover cellular processes. Through viral studies we have recently identified a novel translational control mechanism that involves the DEAD-box helicase Dhh1/DDX6 and RNA folding within coding sequences (CDSs). All Dhh1-dependent mRNAs, viral and cellular ones, (i) contain long and highly structured CDSs, (ii) are directly bound by Dhh1 with a specific pattern, (iii) are activated at the translation initiation step and (iv) express proteins associated with the endoplasmic reticulum. The obtained results uncover a novel layer of translation regulation associated with translation at the endoplasmic reticulum conserved from yeast to humans and hijacked by viruses.
病毒是揭示细胞过程的有力工具。通过病毒研究，我们最近发现了一种新的翻译控制机制，该机制涉及DEAD-box解旋酶Dhh1/DDX6以及编码序列（CDS）内的RNA折叠。所有依赖Dhh1的mRNA，包括病毒和细胞来源的，（i）含有长且高度结构化的CDS，（ii）以特定模式直接与Dhh1结合，（iii）在翻译起始步骤被激活，并且（iv）表达与内质网相关的蛋白质。所获得的结果揭示了一层新的翻译调控，它与内质网上的翻译相关，从酵母到人类均保守存在，并被病毒利用。
