钠钾ATP酶二维膜晶体的组装

Assembly of two-dimensional membrane crystals of Na,K-ATPase.

作者信息

Söderholm M, Hebert H, Skriver E, Maunsbach A B

机构信息

Department of Medical Biophysics, Karolinska Institutet, Stockholm, Sweden.

出版信息

J Ultrastruct Mol Struct Res. 1988 Jun;99(3):234-43. doi: 10.1016/0889-1605(88)90067-5.

DOI:10.1016/0889-1605(88)90067-5

PMID:2848906

Abstract

The assembly of vanadate-induced two-dimensional membrane crystals of Na,K-ATPase was analyzed by electron microscopy and image processing. Electron micrographs of negatively stained linear arrays of protein molecules were recorded and processed by correlation averaging methods. The arrays were compared with fully developed p21 crystals of the enzyme. On the basis of similarity in protein form, symmetry, and packing arrangement it was concluded that the fully developed crystals are built of tightly packed ribbons. Assembly pathways for two-dimensional membrane crystals of Na,K-ATPase are proposed.

摘要

通过电子显微镜和图像处理分析了钒酸盐诱导的钠钾ATP酶二维膜晶体的组装。记录了蛋白质分子负染色线性阵列的电子显微照片，并通过相关平均法进行处理。将这些阵列与该酶完全发育的p21晶体进行比较。基于蛋白质形态、对称性和堆积排列的相似性，得出完全发育的晶体由紧密堆积的条带构成的结论。提出了钠钾ATP酶二维膜晶体的组装途径。