Herbert H, Skriver E, Maunsbach A B
FEBS Lett. 1985 Jul 22;187(1):182-6. doi: 10.1016/0014-5793(85)81238-2.
The three-dimensional structure of Na,K-ATPase was determined by electron microscopy and image processing. Tilt series of negatively stained membrane crystals were recorded. The projections were analyzed by Fourier methods and the data combined to a 3-D model. The unit cell contains two rod-shaped stain-deficient regions interpreted as alpha beta-promoters of Na,K-ATPase. The rods are related by dyad axes oriented perpendicular to the membrane. Outside the lipid bilayer the rods contact different protein units on the two sides of the membrane.
钠钾ATP酶的三维结构通过电子显微镜和图像处理技术得以确定。记录了负染膜晶体的倾斜系列图像。通过傅里叶方法对投影进行分析，并将数据整合为三维模型。晶胞包含两个杆状的低染色区域，被解释为钠钾ATP酶的αβ亚基。这些杆通过垂直于膜的二元轴相互关联。在脂质双层之外，杆在膜的两侧与不同的蛋白质单元接触。
