Wandscheer Alana C D, Marchesan Enio, Tedesco Solange B, Frescura Viviane Dal-Souto, Soares Camille F, Londero Guilherme P, Teló Gustavo M, Hansel Dâmaris S S
Universidade Federal de Santa Maria, Centro de Ciências Rurais, Departamento de Fitotecnia, Avenida Roraima, 1000, Prédio 85, Cidade Universitária, Camobi, 97105-900 Santa Maria, RS, Brazil.
Universidade Federal de Santa Maria, Centro de Ciências Naturais e Exatas, Departamento de Biologia, Laboratório de Citogenética Vegetal e Genotoxicidade, Avenida Roraima, 1000, Prédio 16, Sala 3111, Cidade Universitária, Camobi, 97105-900 Santa Maria, RS, Brazil.
An Acad Bras Cienc. 2017 Apr-Jun;89(2):1251-1258. doi: 10.1590/0001-3765201720150536. Epub 2017 May 2.
Tricyclazole is currently one of the fungicides recommended for the treatment of diseases in irrigated rice. However, there is relatively little information on its cytotoxic and genotoxic potential. The objective of this study was to evaluate the cytotoxicity and genotoxicity of rice crop water after apllication of the tricyclazole fungicide through the Allium cepa L. test. The rice crop water samplings were collected before and 1, 15 and 30 days after application of the fungicide in rice plant shoots. The Allium cepa roots were placed in contact with the rice crop water to check for possible chromosomal abnormalities and mitotic index of the bioindicators meristematic cells. The data obtained by the Allium cepa test indicates that the application of the tricyclazole fungicide leads to an increase in the genotoxic activity in the rice crop water, through the appearance of chromosomal abnormalities, without, however, causing significant effects on the mitotic index. The major chromosomal alterations observed were anaphasic and telophasic bridges and laggard chromosomes.
三环唑是目前推荐用于防治灌溉稻田病害的杀菌剂之一。然而，关于其细胞毒性和遗传毒性潜力的信息相对较少。本研究的目的是通过洋葱（Allium cepa L.）试验评估三环唑杀菌剂施用后稻田水的细胞毒性和遗传毒性。在水稻植株地上部施用杀菌剂之前以及施用后1天、15天和30天采集稻田水样。将洋葱根尖与稻田水接触，以检查生物指示分生细胞的可能染色体异常和有丝分裂指数。通过洋葱试验获得的数据表明，三环唑杀菌剂的施用导致稻田水中遗传毒性活性增加，表现为出现染色体异常，但对有丝分裂指数没有显著影响。观察到的主要染色体改变是后期和末期桥以及落后染色体。
