Wang Yuchuan, Lin Jinyong, Chen Luxia, Wang Liming, Hao Peng, Han Ruifang, Ying Ming, Li Xuan
*Department of Genetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; and †Tianjin Key Laboratory of Ophthalmology and Visual Science, Tianjin Eye Hospital, Tianjin Eye Institute, Nankai University Eye Hospital, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
Cornea. 2017 Jul;36(7):841-844. doi: 10.1097/ICO.0000000000001213.
The expression of peroxiredoxin 2 and vascular endothelial growth factor receptor 2 (VEGFR2) was detected in pterygium to investigate whether they are involved in the pathogenesis or recurrence of pterygium and to evaluate the association between peroxiredoxin 2 and VEGFR2 in pterygium.
Ten normal bulbar conjunctivae, 35 primary pterygia, and 35 recurrent pterygia were obtained. Formalin-fixed, paraffin-wax-embedded tissues were analyzed by immunohistochemistry with peroxiredoxin 2 and VEGFR2 antibodies.
There was no statistical difference between primary pterygia and recurrent pterygia in terms of age and sex (P = 0.685; P = 0.811). The expression rate of peroxiredoxin 2 (94.3%, 66/70) and VEGFR2 (61.4%, 43/70) was increased in pterygia compared with normal conjunctivae (negative). The expression of peroxiredoxin 2 in recurrent pterygia (negative 0, weak 0, moderate 27, strong 8) was higher than that in primary pterygia (negative 6, weak 16, moderate 13, strong 0) (P < 0.001). The expression of VEGFR2 in recurrent pterygia (negative 4, weak 5, moderate 12, strong 4) was higher than that in primary pterygia (negative 23, weak 10, moderate 1, strong 1) (P < 0.001). The expression of peroxiredoxin 2 was consistent with that of VEGFR2 in pterygium (r = 0.348, P = 0.006).
Overexpression of peroxiredoxin 2 and VEGFR2 in pterygium might be involved in the pathogenesis or recurrence of pterygium. The increase of VEGFR2 might be related to the increase of peroxiredoxin 2 in response to excessive reactive oxygen species from ultraviolet exposure.
检测翼状胬肉中过氧化物酶体增殖物激活受体2（Prx2）和血管内皮生长因子受体2（VEGFR2）的表达，探讨它们是否参与翼状胬肉的发病机制或复发，并评估翼状胬肉中Prx2与VEGFR2之间的关联。
获取10例正常球结膜、35例原发性翼状胬肉和35例复发性翼状胬肉。用Prx2和VEGFR2抗体通过免疫组织化学分析福尔马林固定、石蜡包埋的组织。
原发性翼状胬肉和复发性翼状胬肉在年龄和性别方面无统计学差异（P = 0.685；P = 0.811）。与正常结膜（阴性）相比，翼状胬肉中Prx2的表达率（94.3%，66/70）和VEGFR2的表达率（61.4%，43/70）升高。复发性翼状胬肉中Prx2的表达（阴性0，弱阳性0，中度27，强阳性8）高于原发性翼状胬肉（阴性6，弱阳性16，中度13，强阳性0）（P < 0.001）。复发性翼状胬肉中VEGFR2的表达（阴性4，弱阳性5，中度12，强阳性4）高于原发性翼状胬肉（阴性23，弱阳性10，中度1，强阳性1）（P < 0.001）。翼状胬肉中Prx2的表达与VEGFR2的表达一致（r = 0.348，P = 0.006）。
翼状胬肉中Prx2和VEGFR2的过表达可能参与翼状胬肉的发病机制或复发。VEGFR2的增加可能与Prx2因紫外线暴露产生的过量活性氧增加有关。
