Han Yun-Fei, Liu Zhen, Wang Bang, Zhu Wei, Li Jing-Zhen, Qi Yue-Qin, Li Xiao-Jing, Xu Yan-Yun, Dou Xiao-Xiao, Mu Guo-Ying
Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
Aier Eye Hospital Group, Hubin Aier Eye Hospital, Binzhou 256600, Shandong Province, China.
Int J Ophthalmol. 2019 Jun 18;12(6):892-897. doi: 10.18240/ijo.2019.06.02. eCollection 2019.
To investigate the relationship between semaphorin 7a expression and cell proliferation and migration in pterygium fibroblasts.
Twenty-six patients with surgically diagnosed pterygium were enrolled, including 15 cases of primary pterygium and 11 cases of recurrent pterygium. In addition, 12 cases of normal conjunctival tissue were collected. The expression of semaphorin 7a in normal conjunctival tissue, primary pterygium and recurrent pterygium was detected by real-time polymerase chain reaction. Recurrent pterygium fibroblasts were isolated and cultured, and the expression of semaphorin 7a was silenced by small interfering RNA (siRNA) interference technique. Furthermore, the effects of si-semaphorin 7a interference on the mRNA and protein levels of β1-integrin, vascular endothelial growth factor A (VEGFA) and vascular endothelial growth factor receptor (VEGFR), and on fibroblast proliferation were analyzed. Transwell assay was used to detect the effect of semaphorin 7a interference on fibroblast migration.
Semaphorin 7a was highly expressed in the primary pterygium and recurrent pterygium samples than that of the normal conjunctival tissue. Compared with the primary pterygium, the expression of semaphoring 7a in the recurrent pterygium samples was significantly increased (<0.05). The mRNA and protein expression levels of β1-integrin, VEGFA and VEGFR were decreased after si-semaphorin 7a transfection, and as well as the cell proliferation and migration.
Semaphorin 7a might play important roles in the pathogenesis of pterygium by affecting the expression of β1-integrin, VEGFA and VEGFR.
探讨信号素7a表达与翼状胬肉成纤维细胞增殖及迁移之间的关系。
纳入26例经手术诊断为翼状胬肉的患者，其中原发性翼状胬肉15例，复发性翼状胬肉11例。此外，收集12例正常结膜组织。采用实时聚合酶链反应检测正常结膜组织、原发性翼状胬肉和复发性翼状胬肉中信号素7a的表达。分离并培养复发性翼状胬肉成纤维细胞，采用小干扰RNA（siRNA）干扰技术沉默信号素7a的表达。此外，分析si-信号素7a干扰对β1整合素、血管内皮生长因子A（VEGFA）和血管内皮生长因子受体（VEGFR）的mRNA和蛋白水平以及对成纤维细胞增殖的影响。采用Transwell实验检测信号素7a干扰对成纤维细胞迁移的影响。
信号素7a在原发性翼状胬肉和复发性翼状胬肉样本中的表达高于正常结膜组织。与原发性翼状胬肉相比，复发性翼状胬肉样本中信号素7a的表达显著增加（<0.05）。si-信号素7a转染后，β1整合素、VEGFA和VEGFR的mRNA和蛋白表达水平降低，细胞增殖和迁移也降低。
信号素7a可能通过影响β1整合素、VEGFA和VEGFR的表达在翼状胬肉的发病机制中发挥重要作用。
